Search Results

You are looking at 51 - 60 of 764 items for :

  • All content x
Clear All

Recent findings on the pathogenesis of bronchial asthma

Part II. The role of hormonal predisposition, environmental influences and conditioning leading to bronchial asthma

Acta Physiologica Hungarica
Authors: József Székely and Á. Pataki

120 Belmonte KE, Fryer AD, Costello RW: Role of insulin in antigen-induced airway eosinophilia and neuronal M2 muscarinic receptor dysfunction. J. Appl. Physiol. 85, 708–718 (1998

Restricted access

., 2002 ). There are two forms of insulin-like growth factor (IGF): insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-2 (IGF-2) ( Rinderknecht and Humbel, 1978; Engström et al., 1998 ). The former is expressed after birth and is produced

Restricted access

-glucosidase, which restricts intestinal glucose absorption ( Hou et al., 2009 ), and protein tyrosine phosphatase 1B, which is a key factor in the negative regulation of the insulin pathway and a promising target for the treatment of DM and obesity ( Zhang et al

Restricted access

Carstensen, B., Witte, D. R, Friis, S.: Diabetes and insulin duration and cancer incidence: a register linkage study in Denmark. Diabetologia, 2010, 53 (Suppl. 1) , S67. Friis S. Diabetes

Restricted access

Abstract  

In this article we studied the dynamic dissociation constant (k d) of 99Mo complexed with insulin molecule at various pH. The k d values were determined by dialysis technique against deionised water. The T 1/2 of the molybdenum–insulin complexes were found to be 6.41, 5.25 and 3.5 h at pH 5, 6 and 7 respectively. The half-lives indicate that insulin may act as good carrier of 99Mo to the intestine and may be useful in the field of nuclear medicine.

Restricted access

Abstract  

In order to target insulin receptors in various diabetic and insulinoma conditions, human recombinant insulin was successively labeled with [111In]-indium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (ccDTPA). The best results of the conjugation were obtained by addition of 0.5 mL of an insulin pharmaceutical solution (5 mg/mL, in phosphate buffer, pH 8) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 °C with continuous mild stirring for 30 minutes. Radio-thin layer chromatography (RTLC), instant thin layer chromatography (ITLC) and high performance liquid chromatography (HPLC) have shown an overall radiochemical purity of higher than 93% at optimized conditions (specific activity = 550–750 MBq/mg, radiochemical yield =81%). The white blood cell labeling capacity of the tracer was determined up to 4 hours at 37 °C. Preliminary in vivo studies in normal rat model was performed to determine the biodistribution of the radiotracer up to 48 hours. It showed a high liver and spleen uptake of the tracer which is consistent with other reported radiolabeled insulins. SPECT images have also shown high liver accumulation of the tracer.

Restricted access

Apa, R., Di Simone, N., Ronsisvalle, E., Miceli, F., de Feo, D., Caruso, A., Lazone, A. and Mancuso, S. 1996: Insulin-like growth factor (IGF)-I and IGF-II stimulate progesterone production by human luteal cells

Restricted access
Orvosi Hetilap
Authors: Zsuzsanna Soós, Anna Czégeni, Ferenc Salamon, Mónika Salamon, István Kenessey, András Folyovich, Tibor Tihanyi, and Gábor Winkler

, P.: Insulin secretion and insulin-producing tumors. Expert Rev. Endocrinol. Metab., 2010, 5 , 217–227. Gorden P. Insulin secretion and insulin-producing tumors

Open access

Abstract  

A first attempt to label insulin, a small protein with significant affinity to tumors with the α-emitter 211At was performed by an indirect method using N-succinimidyl 5-(tributylstannyl)-3-pyridinecarboxylate (SPC) as a bi-functional linker, and the stability of the conjugated insulin (211At-insulin) was evaluated in vitro and in vivo. SPC was synthesized by using 5-bromonicotinic acid as the starting material. With this bi-functional linker, insulin was conjugated with 211At in a labeling yield of 30–40%, with radiochemical purity of more than 98%. After 24 hours at room temperature, the radiochemical purity was still more than 95%, implying that 211At-insulin is fairly stable in vitro. Biodistribution of 211At-insulin was investigated in NIH strain mice. 211At accumulated rapidly in the liver post injection, with the maximum uptake of 4.29%I.D/g at 30 minutes, and was mainly excreted by kidney. More importantly, 211At-insulin uptake in some key organs or tissues, especially in thyriod, stomach, lung and spleen, was much less than that of free astatide (211At). This result indicated that 211At-insulin has considerable stability in vivo as well as in vitro.

Restricted access

Benedict, C., Hallschmid, M., Schmitz, K. és mtsai: Intranasal insulin improves memory in humans: superiority of insulin aspart. Neuropsychopharmacology, 2007, 32 , 239–243. Schmitz K

Open access