Authors:KE Nurullahoğlu-Atalık, S Kutlu, H Solak, and R Özen Koca
increasing nitricoxide production, improving inflammation and oxidation, enhancing endothelial progenitor cells migration, and so on which now are referred to as pleiotropic effects ( 4 , 5 , 28 ). Statins are widely used and are the most effective
The rate of nitrogen isotope exchange between NO and HNO3 has been measured as a function of nitric acid concentration of 1.5–4M·1–1. The exchange rate law is shown to beR=k[HNO3]2[N2O3] and the measured activation energy isE=67.78kJ ·M–1 (16.2 kcal·M–1). It is concluded that N2O3 participates in15N/14N exchange between NO and HNO3 at nitric acid concentrations higher than 1.5M·1–1.
The aim of the present study was to investigate the effects of endogenous endothelin on renal excretory function in spontaneously hypertensive rats (SHR) after inhibition of NO synthesis. The effects of non-selective ETA/ETB receptor blockade on L-NAME-induced changes in renal excretory function and blood pressure (BP) were investigated in conscious, SHR and normotensive Wistar rats with implanted catheters in the bladder for urine collection, in the femoral artery for BP registration and in the femoral vein for L-NAME and bosentan administration. L-NAME increased systolic, mean and diastolic BP, diuresis, sodium and chloride excretion (p<0.01) in both normotensive and hypertensive rats but bosentan returned the values of diuresis, sodium and chloride excretion to control level without any changes in BP in normotensive rats. In SHR the effects of L-NAME were reduced after bosentan (p<0.05) but the values of diuresis, sodium and chloride excretion still remained statistically significant as compared to control level despite the fact that bosentan lowered mean and diastolic BP increased due to L-NAME administration. Endogenous endothelins participate in a different manner in the rise of BP and in the changes in renal excretory function that develops after L-NAME-induced NO synthase inhibition in normotensive rats and in SHR.
Authors:L. Costantini, R. Molinari, and N. Merendino
Some studies suggested a positive effect against cardiometabolic diseases of supplementation of alpha-linolenic acid (ALA, C18:3 n-3) rich foods in pathological subjects, even if the total literature is controversial. In order to clarify ALA-rich chia seed action in hypertensive model with the overt pathology and without drug interference, in the present study the biochemical markers of cardiometabolic diseases (endothelin-1, ET-1; nitric oxide, NO; and bradykinin, BK) in Spontaneously Hypertensive Rats (SHRs) were analysed after 5% chia seed dietary supplementation for five weeks, and compared with the staple raw material wheat and corn. At the end of the experimental period, also plasma antioxidant capacity and inflammatory condition were evaluated. Our results showed that the chia seed group was more oxidized. On the other hand, ET-1 significantly decreased in chia seed group, and there was no difference between groups for NO, BK, and the inflammatory C-reactive protein (CRP). In conclusion, some positive effects of chia seed consumption on cardiometabolic markers in SHRs were observed, despite this the association of chia seeds with antioxidants is suggested to avoid plasma oxidation increase.
Authors:Malgorzata Slowinska-Lisowska, A. Zembron-Lacny, M. Rynkiewicz, T. Rynkiewicz, and W. Kopec
Carnosine is a dipeptide formed from the amino acids β-alanine and histidine and found in large amounts in the brain and muscle, especially fast twitch muscle. Carnosine has an antioxidant role and accounts for about 10% of the muscle’s ability to buffer the H+ ions produced by high intensity exercise. Due to the interesting role of carnosine, the aim of the study was observe the effects of carnosine intake on pro-antioxidant status in highly trained athletes exposed to intense exercise.Fourteen male athletes from the Polish national kayak and canoe teams participated in placebo-controlled and cross-over study. The athletes were supplemented with 4 g/d carnosine for 14 days. Blood samples were collected before and 30 min, 24 h and 48 h after 2000 m exercise trial. In blood, hydrogen peroxide (H2O2), nitric oxide (NO), markers of RO/NS activity 8-isoprostanes and 3-nitrotyrosine, total (GSHt) and oxidised glutathione (GSSG), antioxidant status (APO) and superoxide dismutase (SOD) were determined. There were not observed statistically significant differences in exercise-induced changes in H2O2 and NO concentrations and SOD activity after carnosine intake. However, carnosine prevented an increase in 8-isoprostanes, 3-nitrotyrosine and GSSG concentrations as well as elevated redox status (GSHt-2GSSG)/GSSG at post-exercise period.Although, oral supplementation with 4 g carnosine did not affect RO/NS generation, it significantly attenuated exercise-induced glutathione loss, reduced oxidation/nitration markers concentration and SOD activity. These results suggest that carnosine could provide antioxidative protection for highly trained athletes.
Preparation of a Fe-mordenite catalysts was carried out by impregnation using Fe(acac)3 precursor in order to have iron oxide species deposited at the surface of the zeolite. The selective presence of iron oxide species was determined and ascertained by temperature programmed reduction (TPR). In the selective catalytic reduction of NO by ammonia, no difference of conversion between the catalysts was observed indicating that well dispersed iron oxide species are active species for this reaction. Nevertheless, the obtained activity remains lower than catalysts containing iron cationic species at the exchange sites.