Authors:M Ninković, M Ninković, M Ninković, Z Maličević, Z Maličević, Z Maličević, A Jelenković, A Jelenković, A Jelenković, DM Jovanović, DM Jovanović, DM Jovanović, M Đukić, M Đukić, M Đukić, I Vasiljević, I Vasiljević, and I Vasiljević
a part of blood-brain barrier, brain capillaries participate in
pathophysiological events during systemic inflammation. We investigated the
effects of 7-nitroindazole (7-NI), selective neuronal nitric oxide synthase
(NOS) inhibitor, to oxidative status (OS) of brain capillaries. Adult Wistar
rats were randomized at groups: control group (CG) (sham operated), sepsis
group (GS) (cecal ligation and perforation with inoculation of Escherichia
coli (ATCC 25922), 7-NI group (G7-NI), (30 mg/kg b/w i. p.) and 7-NI +
sepsis group (G7-NIS), (7-NI was applied 30 minutes before operation). Lipid
peroxidation index (LPI), nitrite concentration, superoxide dismutase (SOD)
activity and superoxide anion (O2·-) content were determined 3, 6, 24 and 48 hour in each
group. Cerebral capillaries were separated from non-vascular brain tissue
using sucrose gradient. Compared to controls, LPI, nitrite and O2·- increased at SG. In the G7-NIS, LPI reached control
values at the 24 th and 48 th hour, while nitrite were
decreased at the 3 rd and 24 th hour, compared to controls. In the same
group, O2·- decreased at the 3 rd, 6 th and 24 th hour, although SOD showed variable activity.
The systematic nNOS inhibition with 7-NI forces OS on early terms of sepsis,
but lately it contributes to the normalization of OS in cerebral capillaries.
Authors:H.Q. Zhao, L. Wang, J. Hong, X.Y. Zhao, X.H. Yu, L. Sheng, C.Z. Hang, Y. Zhao, A.A. Lin, W.H. Si, and F.S. Hong
Salt stress impaired Mn imbalance and resulted in accumulation of ROS, and caused oxidative stress to plants. However, very little is known about the oxidative damage of maize roots caused by exposure to a combination of both salt stress and Mn deprivation. Thus the main aim of this study was to determine the effects of a combination of salt stress and Mn deprivation on antioxidative defense system in maize roots. Maize plants were cultivated in Hoagland’s media. They were subjected to 80 mM NaCl administered in the Mn-present Hoagland’s or Mn-deficient Hoagland’s media for 14 days. The findings indicated that the growth and root activity of maize seedlings cultivated in a combination of both salt stress and Mn deprivation were significantly inhibited; the compatible solute accumulation, malondialdehyde, carbonyl, 8-OHdG, and ROS were higher than those of the individual salt stress or Mn deprivation as expected. Nevertheless, the antioxidative enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase, glutathione-S-transferase and antioxidants such as ascorbic acid, glutathione and thiol were lower than those of the individual salt stress or Mn deprivation. In view of the fact that salt stress impaired Mn nutrition of maize seedlings, the findings suggested that Mn deprivation at the cellular level may be a contributory factor to salt-induced oxidative stress and related oxidative damage of maize roots.
Authors:S. Hiriyanna Hiriyanna, K. Basavaiah, P. S. Goud Goud, V. Dhayanithi, K. Raju, and H. Pati Pati
Studies of the degradation of olanzapine bulk drug under hydrolytic (acidic and alkaline), oxidising, and photolytic conditions are reported in this paper. Olanzapine is almost completely stable under photolytic and hydrolytic (acidic and alkaline) conditions. The major degradation products formed when olanzapine bulk drug was exposed to oxidative stress conditions were isolated by preparative reversed-phase high-performance liquid chromatography and characterized by FTIR, LC-MS-MS, and 1H, 13C, and DEPT NMR. On the basis of results from spectroscopy the degradation products were characterized as 10-hydroxy-2-methyl-5,10-dihydro-4H-benzo[b]thieno [2,3-e][1,4]diazepin-4-one, 2-methyl-5,10-dihydro-4H-benzo[b]thieno[2,3-e][1,4]diazepin-4-one, and 2-methyl-10-(2-methyl-10H-benzo[b]thieno[2,3-e][1,4] diazepin-4-yloxy)-5,10-dihydro-4H-benzo[b]thieno[2,3-e]diazepin-4-one. Identification, isolation, and characterization of these oxidative degradation products are discussed in detail.
Authors:Y. Sakuma, K. Matsuoka, C. Honda, K. Matsumoto, and K. Endo
The dynamics of redox related elements (Fe, Co, Zn, and Se) were studied using instrumental neutron activation analysis as
a function of rat age in the range of 4 to 16 weeks. Activity levels of glutathione peroxidase (GSH-Px), thiobarbituric acid
reactive substance (TBARS) were assayed, and hydrogen peroxide (H2O2) concentrations were measured for the same liver homogenates using an X-band ESR spectrometer. The oxidative stress, the
aging effect, and the mineral valance are discussed.