Authors:B. Ivković, J. Brborić, V. Dobričić, and O. Čudina
The analysis of preservatives in commercial pharmaceutical products is particularly important for both quality assurance and consumer protection. International Conference on Harmonization (ICH) guidance recommends
An HPTLC method, using an internal standard, for analysis of colchicine in a pharmaceutical formulation, has been established and validated. The analyte and internal standard were separated on aluminum plates precoated with silica gel 60 F
; the mobile phase was ethyl acetate-acetonitrile-water-formic acid 8.0:1.0:0.5:0.5 (
). Quantification was by densitometric scanning at 358 nm. Response was a linear function of colchicine concentration in the range 5 to 35 μg mL
. The limits of detection and quantification for colchicine were 1 and 5 μg mL
, respectively. Average recovery of colchicine was 100.48%, which showed the method was free from interference from excipients present in the formulation. The established method enabled accurate, precise, and rapid analysis of colchicine in the pharmaceutical formulation.
A simple and precise reversed-phase thin-layer chromatographic (RP TLC) method for simultaneous separation of fluoxetine and citalopram in pharmaceutical preparations has been developed and validated. Separation was performed on RP-18 F254 TLC plates with methanol-0.05 M phosphate buffer (pH 5)-triethylamine 68:27:5 (v/v) as mobile phase. Densitometric detection was performed at 230 nm. The method was validated for linearity, accuracy, precision, selectivity, and robustness. Calibration plots showed the response, as peak area, was a linear (r2 > 0.9988) function of the amounts of the compounds in the concentration range 500–5000 ng per spot. Statistical analysis proved the method was both precise and accurate. The method was successfully used for analysis of citalopram and fluoxetine in their pharmaceutical preparations, with recovery of the compounds ranging from 99.10 to 101.70% of the labeled amount.
This study is a count of the publications of a sample of the major pharmaceutical multinational companies. These firms have been divided into three geopolitical groups: Europe, the United States and Japan. Results obtained show that research activities in this industry have been subjected to some changes between 1965 and 1979. Among these changes is the growing importance of fundamental research, the erosion of the leadership of U.S.-based firms and the growing importance of overseas research.
Authors:Małgorzata Starek, Jan Krzek, and Marzena Stoch
A TLC-densitometric method has been established for identification and quantification of tiaprofenic acid, ketoprofen, naproxen, dexibuprofen, flurbiprofen, alminoprofen, and ibuprofen in selected pharmaceutical preparations. The separation was performed on TLC silica gel F
plates using two mobile phases. UV densitometry was performed at 225 and 270 nm. The method is of high sensitivity; limits of detection and quantitation range from 0.05 to 0.29 μg per band. For individual constituents recovery ranged from 98.71% to 102.65%.
50 pharmaceutical patents granted to firms, residing in US, GB, DE and HU each, were surveyed and the average numbers of scientific as well as patent itemsReferenced by the inventors were calculated. The sum of impact factors of the journals referenced (Total Weighted Impact) was calculated by scientific fields. About 50–60 per cent of scientific information referred to in the patents was found to originate from Life Sciences journals. It was found that 10 per cent of the journals referenced contained 55 per cent of the papers.