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. FAO, 2005: FAOSTAT data, http://faostat.fao.org Last updated February 2005 Kőszegi, Kovács (2004): Investigations on the plant structure characteristics of emmer landraces under inhomogeneous conditions, Acta

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Cereal Research Communications
Authors: M.Z. Islam, M.A. Siddique, N. Akter, M.F.R.K. Prince, M.R. Islam, M. Anisuzzaman, and M.A.K. Mian

( Oryza sativa l. ) landraces in Bangladesh . Bangladesh J. Pl. Breed. Genet. 2 : 33 – 40 . Athwal , D.S. , Virmani , S.S. 1972 . Cytoplasmic male sterile and hybrid

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Bucheyekei, T.L., Gwanama, C., Mgonja, M., Chisi, M., Folkertsma, R., Mutegi, R. 2009. Genetic variability characterisation of Tanzania sorghum landraces based on Simple Sequence Repeats (SSR) molecular and morphological markers. Afr. J. Crop Sci. 17 :71

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Davood, A., Ashkboos, F., Sadeghi, M., Bahram, D.N. 2004. Evaluation of salinity tolerance in landrace wheat germplasms of Cereals Research Collection Department. Isfahan Agricultural and Natural Resources Research Center Publisher, Isfahan, Iran, 14 pp

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Acta Veterinaria Hungarica
Authors: Marta Jackowska, Bartosz Kempisty, Magdalena Woźna, Hanna Piotrowska, Paweł Antosik, Piotr Zawierucha, Dorota Bukowska, Michał Nowicki, Jędrzej Jaśkowski, and Klaus-Peter Brüssow

The TGFB superfamily genes are involved in several important cell functions, including proliferation and differentiation, and the role of the expression of these genes in growth and development of theca and granulosa cells is well recognised. However, the dependence between the stage of oocyte maturation or follicular size and the expression of these genes in pigs is still not entirely known. This study was aimed at investigating the expression pattern of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes before and after in vitro maturation (IVM) as well as in oocytes collected from follicles of different sizes. RQ-PCR was performed to analyse the expression of GDF9, TGFB1, TGFB2 and TGFB3 in oocytes before and after IVM (oocytes cultured for 44 h in TCM-199), isolated from large (> 5 mm), medium (3–5 mm) and small (< 3 mm) follicles collected from ovaries of 28 puberal crossbred Landrace gilts after slaughter. We found an increased expression of both TGFB1 and TGFB2 in oocytes before IVM collected from large as compared to medium and small follicles (P < 0.05, P < 0.001, P < 0.01, P < 0.05, respectively). In these groups of oocytes we did not observe differences in GDF9 and TGFB3 mRNA levels. However, after IVM, GDF9 protein distribution in oocytes was significantly higher in large and medium follicles as compared to small ones (P < 0.01, P < 0.001, respectively). Moreover, an increased TGFB1, TGFB2 and TGFB3 proteins pattern was observed in oocytes of large compared to small follicles. The highest GDF9 and TGFB1 mRNA levels were found in oocytes after IVM compared to those before IVM. Based on our study we can suppose that the distribution pattern of TGFB superfamily genes is associated with the stage of maturation of porcine oocytes and the follicle size. Furthermore, GDF9 and TGFB1 may serve as molecular markers of the develop-mental potential of porcine oocytes. The confocal microscopic observation revealed that TGFB1 and TGFB3 were translocated between the zona and the cytoplasm of oocytes, depending on the stage of maturation and follicle size.

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2002 Stelmakh, A.F. 1990. Geographic distribution of Vrn genes in landraces and improved varieties of spring bread wheat. Euphytica 45 :113

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Kőszegi, B., Kovács, G. (2003): Gradient chamber studies on the dry matter accumulation of winter emmer [ Triticum turgidum ssp. dicoccon (Schrank) Thell.] landraces in the seedling stage. Acta Agron. Hung. , 51 , 413

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Acta Agronomica Hungarica
Authors: M. Hudcovicová, V. Šudyová, S. Šliková, E. Gregová, J. Kraic, F. Ordon, D. Mihálik, V. Horevaj, and Z. Šramková

Gregová, E., Hermuth, J., Kraic, J., Dotlačil, L. (1999): Protein heterogeneity in European wheat landraces and obsolete cultivars. Genetic Resources and Crop Evolution , 46 , 521–528. Dotlačil L

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Peter R. — Eschholz T.W. — Stamp P — Liedgens M.: 2006. Swiss maize landraces — Early vigour adaptation to cool condition — Acta Agronomica Hungarica vol. 54 no. 3 329–336 pp. Liedgens M

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Ahmed, I. 2004. Genetic Diversity for Agro-morphological and High Molecular Weight Glutenin Subunits in Wheat ( Triticum aestivum L.) Landraces. PhD dissertation. University of Arid Agriculture, Rawalpindi

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