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Cereal Research Communications
Authors: I. Baracskai, G. Balázs, L. Liu, W. Ma, M. Oszvald, M. Newberry, S. Tömösközi, L. Láng, Z. Bedő, and F. Békés

://www.aaccnet.org/grainbin/pdfs/II_HMW_Subunits.pdf Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, W., Wrigley, C.W. 2006c. The Gluten Composition of Wheat Varieties and Genotypes. Part II. Composition table for LMW-GS. http://www.aaccnet.org/grainbin/pdfs/III_LMW Subunits

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Branlard, G., Dardever, M., Amiour, N., Igrejas, G. 2003. Allelic diversity of HMW and LMW glutenin subunits and omega-gliadin in French wheat ( Triticum aestivum L.). Genetic Resources and Crop Evolution 50 :669

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Bradova, J. 2008. Allelic diversity of HMW- and LMW-glutenin subunits wheat varieties ( Triticum aestivum L.) registered in the Czech Republic. In: Prohens, J., Badenes, M.L. (eds), Modem Variety Breeding for Present and Future Needs. Proceedings of

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Cereal Research Communications
Authors: S. Wang, D. Chen, G. Guo, T. Zhang, S. Jiang, X. Shen, D. Perovic, S. Prodanovic, and Y. Yan

In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.

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The genetic variations of high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) as well as of ω- and γ-gliadins in 562 accessions of 7 tetraploid Triticum turgidum L. subspecies were investigated using sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE). A total of 26 HMW-GS alleles (7 at Glu-A1 and 19 at Glu-B1 loci) with 63 allelic combinations, as well as 11 LMW-GS alleles (5 at Glu-A3, 4 at Glu-B3 and 2 at Glu-B2 loci) with 26 allelic combinations, were detected. Two novel HMW-GS, called B1cf and B1cg, were discovered in T. dicoccum, B1cg was also found in T. turanicum. The Glu-B1 locus showed the highest values of genetic diversity index (H), with a mean of 0.72. As regards gliadins, 8 alleles at Gli-B1 locus have been found. The dendrogram based on allelic frequencies, revealed that T. durum, T. carthlicum and T. polonicum grouped a part from the other subspecies. This behaviour suggested probably different evolutive pathways among the tetraploid wheats.

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Two hundred and forty diverse set of wheat cultivars released in India during the last several decades were evaluated for HMW and LMW glutenin alleles, for assessing their diversity and effect on sedimentation volume and mixograph parameters. Both SDS-PAGE and PCR based markers were employed in identifying alleles encoded at Glu-1 and Glu-3 loci. Extensive allelic variation was observed at both the Glu-1 and Glu-3 loci. There was prevalence of Glu-A1b, Glu-B1i, Glu-D1a, Glu-A3c, Glu-B3b, Glu-B3g and Glu-D3b. The alleles Glu-A1b, Glu-B1i, Glu-D1d, Glu-A3b, Glu-B3g/h and Glu-D3b exhibited high SDS-sedimentation volume. Glu-B1i and Glu-D1d showed highly significant positive effect (p < 0.001) on sedimentation volume and also had additive effects. However, surprisingly overall there was decline in the frequency of Glu-B1i allele during last two decades in Indian wheat breeding and not a single 1B/1R translocation cultivar possessed this allele. Glu-A1b showed significant positive effect on mixograph peak time, peak slope and peak width. Glu-B3g exhibited significantly higher mixograph peak time and width at 8 and Glu-B3h showed higher dough stability. Glu-B3j (1B/1R translocation) exhibited highest peak slope indicating the negative effect on dough strength. This information can be useful in designing breeding program for the improvement of Indian bread wheat quality.

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Maartens, H. 1999. The inheritance and genetic expression of LMW Glutenin sub-units in South African wheat cultivars. Ph.D. Dissertation. University of Orange Free State. Bloemfontein, South Africa. Maartens H

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Autran J.C., Laignelet B., Morel M.H. 1987. Characterization and quantification of LMW glutenins in durum wheats. Biochimie 69 :699–711. Morel M

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., Simeone, M., Masci, S., Porceddu, E. 1997. Molecular characterization of a LMW-GS gene located on chromosome 1B an the development of primers specific for the Glu-B3 complex locus in durum wheat. Theor. Appl. Genet. 95 :1119

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. Relationship of HMW, LMW glutenin subunits and gliadins with gluten strength in Indian durum wheats. J. Plant Biochem. Biotech. 13 :51–55. Bhosale S.B. Relationship of HMW, LMW glutenin

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