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half of biopsies were processed for gene expression analysis. Briefly, bone biopsies were homogenized with mortar, pestle, and liquid nitrogen, and centrifuged at 2000 rpm for 7 min. Trizol (Fermentas, ON, Canada) was used for RNA isolation. cDNA was

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Interventional Medicine and Applied Science
Authors: Sergei N. Danilchenko, Aleksei N. Kalinkevich, Roman A. Moskalenko, Vladimir N. Kuznetsov, Aleksandr V. Kochenko, Evgenia V. Husak, Vadim V. Starikov, Fuyan Liu, Junhu Meng, and Jinjun Lü

elements in the “labile” (water soluble) state in contrast with the bulk content available from energy-dispersive spectroscopy (EDS). For AS analysis, the material of deposit was annealed at a temperature of 200 °C, homogenized by grinding in a mortar, and

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Acta Microbiologica et Immunologica Hungarica
Authors: Szabolcs Vigvári, Dávid Sipos, Jenő Solt, Áron Vincze, Béla Kocsis, Zsuzsanna Nemes, Ágnes Kappéter, Zsófia Feiszt, Beáta Kovács, and Zoltán Péterfi

within 2 h of passage. A 60-g sample was homogenized in a mortar and suspended in 200 ml of normal saline (0.9%). The suspension was then filtered through 4 × 4 cm sheets of sterile gauze into another container, and 100 ml of the filtrate was acquired in

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Acta Microbiologica et Immunologica Hungarica
Authors: Szabolcs Vigvári, Áron Vincze, Jenő Solt, Dávid Sipos, Zsófia Feiszt, Beáta Kovács, Ágnes Kappéter, and Zoltán Péterfi

the cases. The donated feces were collected and transported to our department on the day of the planned FMT. A 60-g sample was homogenized in a mortar and suspended in 200 ml of normal saline (0.9%). The suspension was then filtered through 4 × 4 cm

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Acta Microbiologica et Immunologica Hungarica
Authors: Flóra Sebők, Csaba Dobolyi, Dóra Zágoni, Anita Risa, Csilla Krifaton, Mátyás Hartman, Mátyás Cserháti, Sándor Szoboszlay, and Balázs Kriszt

incubation and grounded using mortar and pestle. Aliquot of 1 g of the grounded kernels was placed in a test tube and 4 ml of 70% methanol was added. The content of the test tube was mixed for 2 h on a reciprocating shaker (180 rpm) and then centrifuged at 14

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Physiology International
Authors: N. Almási, Z. Murlasits, A. Al-awar, Á. Csonka, S. Dvorácskó, C. Tömböly, S. Török, D. Bester, A. Pósa, C. Varga, and K. Kupai

mortar and pestle, in the case of UCHL-1 the powder was suspended in RIPA buffer (50 mM Tris, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton x-100, 150 mM NaCl); for the detection of eNOS 1 mM Na 3 VO 4 was added to the RIPA buffer. The homogenates were

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