Authors:Christina Grothusen, Tim Attmann, Christine Friedrich, Sandra Freitag-Wolf, Nils Haake, Jochen Cremer, and Jan Schöttler
This study investigated factors determining the long-term outcome and quality of life of patients with a prolonged intensive care unit (ICU) stay after cardiac surgery.
A retrospective analysis was performed in 230 patients that had undergone cardiac surgery and suffered from a post-operative ICU stay of 7 or more days at our institution. Among 11 pre-, 13 intra-, and 14 post-operative variables, factors influencing 5-year outcome were identified by logistic regression analysis. Quality of life was determined using the Short Form-36 questionnaire.
In-hospital mortality was 12%. One hundred and eleven of 187 patients (59%) were alive after 5 years. Non-survivors were older (70 vs. 65 years, p=0.005) and had a higher additive EuroSCORE (7 vs. 5, p=0.034). Logistic regression identified pre-operative atrial fibrillation (AF), (28 vs. 10%, p=0.003) as the strongest predictor for a 5-year outcome, followed by myocardial infarction (62 vs. 41%, p=0.005), and prolonged mechanical ventilation (8 vs. 5 days, p=0.036). Survivors did not show an impaired physical component summary SF-36 score (39 vs. 46, p=0.737) or mental component summary score (55 vs. 55, p=0.947) compared to an age-matched German Normative Sample.
Pre-operative AF proved to be the most important factor determining the 5-year outcome of patients with a prolonged ICU stay after cardiac surgery. Neither physical nor mental health appeared to be impaired in these patients.
Authors:Fatema Moni Chowdhury, Mohammed Ziaur Rahman, Md Murshed Hasan Sarkar, Fazle Rabbi, Sirajul Islam Khan, Chowdhury Rafiqul Ahsan, and Nils-Kåre Birkeland
Recently, we reported the induction of protective immunity by environmental Escherichia albertii strain DM104 against Shigella dysenteriae in guinea pig model. In this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum IgG and mucosal IgA antibody titers. Protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs against live S. dysenteriae. It was found that intranasal immunization showed promising results in terms of antibody response and protective efficacy. All these results reconfirm our previous findings and additionally point out that the intranasal immunization of the environmental E. albertii strain DM104 in guinea pig model can be a better live vaccine candidate against shigellosis.
Authors:Mona Abdel Fattah Razin, Ahmed Osman, Mohamed Ahmed Ali, Mahmoud Mohamed Bahgat, and Amany Sayed Maghraby
-free embryonated chicken eggs (SPF-ECE; Nil S.P.F., Fayoum, Egypt). The virus particles were chemically inactivated by mixing the allantoic fluid with β-propiolactone. Complete inactivation of any infectious viral particles was further confirmed by co