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and retrogradely perfused at the constant pressure of 70 cm H 2 O. The composition of the non-recirculating Krebs–Henseleit perfusate was as follows (in mmol/l): NaCl 118, KCI 4.7, CaCl 2  × 2H 2 O 2.5, MgSO 4  × 7H 2 O 1.7, NaHCO 3 25, KH 2 PO 4 1

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