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asymmetric PCR and its uses in quantitative real-time analysis. PNAS 2004; 101: 1933–1938. 35 Hansen RD, Sorensen M, Tjonneland A, et al. XPA A23G, XPC Lys939Gln

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molecular mechanism of the subjects. Authors’ contribution MS contributed to data collection and/or processing. ATA contributed in concept preparation. AS performed supervision and provided critical review. FK performed real-time

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, Vicaut E , Qualitative real-time analysis by nurses of sublingual microcirculation in intensive care unit: the MICRONURSE study . Crit Care 2015 Nov 6 ; 19 : 388 . https://doi.org/10.1186/s13054-015-1106-3 . PMID: 26542952; PMCID: PMC4635966

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performed in triplicate. The threshold cycle (Ct) of each well and data acquisition were carried out using a software program from Real-Time Analysis Software Programme (Light Cycler ® 480 SW 1.5.0 SP4). The delta Ct (ΔCt) method was used for PCR single

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