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– 24 . E l -A gaimy , M.A. , N eff , W.E. , E l -S ayed , M. A watif , I.I. ( 1994 ): Effect of saline irrigation water on olive oil composition . J. Am. Oil Chem. Soc. , 71 , 1287 – 1289

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bacterial cells were re-suspended in phosphate-buffered saline solution (PBS) and adjusted to the required concentration. Antibacterial activity experiments To assess the ability of K/rGO-CS matrix to capture and kill bacteria, each substrate was immersed in

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treatment ,” Renew. Sust. Energy Rev. , vol. 4 , no. 2 , pp. 135 – 156 , 2000 . 10.1016/S1364-0321(99)00014-3 [9] C. O. Letelier-Gordo , E. Mancini , P. B. Pedersen , I. Angelidaki , and I. A. Fotidis , “ Saline fish wastewater in biogas

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], increased salinity in the ground water and the reduction in the ground water table [ 3 ]. Hence, researchers are trying to find alternate fine aggregate materials. There are limited findings on replacing the fine aggregate completely [ 4, 5 ]. However

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Acta Alimentaria
Authors: H. Soltanmoradi, M.S. Maniati, A. Davoodabadi, A. Mosapour, S. Samavarchi Tehrani, M. Pazhoohan, F. Daemi, and H. Khaleghzadeh-Ahangar

supplement, L. rhamnosus GG, and saline as a control group were used separately to investigate their effect on depression-like behaviour in each group of depressed mice ( n = 8). A fluoxetine-treated group was used as a positive control group. The exact

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Zygosaccharomyces lentus is a significant, recently discovered new osmophilic spoilage yeast, capable of growth at low temperatures. It is remarkably resistant to commonly used preservatives above the legally permitted levels. Therefore, antimicrobial effect of chitosan, cinnamic acid, carvacrol and cymene as new preservatives was tested against Z. lentus in phosphate buffered saline at pH 7.3. Z. lentus was resistant to the antimicrobial action of chitosan (0.5 and 1.0%) and cinnamic acid (0.5–2.0 mM) but was sensitive to the biocidal action of 1.5 and 2.0 mM cymene and 1.5 and 2.0 mM carvacrol in pH-neutral buffer. Further work would need to be done to check the sensitivity of this organism to these compounds at other pH values and temperatures.

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The objective of this work was to study the effect of nisin, alone or combined with sucrose laurate ester (SL), on enhancing Bacillus amyloliquefaciens Fad 82 spores inactivation by pressure-assisted thermal processing (PATP). Spores of B. amyloliquefaciens (108 CFU ml−1) were suspended in sterile phosphate buffer saline (pH 7.6) with concentrations of nisin (0, 100, 200, 1000, 1500 and 2000 IU ml−1) and 1.0% of SL, and combinations of nisin (1000, 1500 and 2000 IU ml−1) and SL (1.1%), and treated by PATP (700 MPa and 105 °C, for 1 min). Nisin did not show any synergetic effect with PATP in all the concentrations tested. Instead, 1.0% of SL alone was effective in enhancing inactivation.

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Acta Alimentaria
Authors: S. Valcheva-Kuzmanova, M. Eftimov, R. Tashev, L. Yankova, I. Belcheva, and S. Belcheva

The main bioactive substances in Aronia melanocarpa fruit juice (AMFJ) are polyphenols (flavonoids, procyanidins, and phenolic acids). A great number of polyphenols are able to traverse the blood-brain barrier. In recent years more attention is drawn to the ability of these substances to influence central nervous system functions. The aim of the present study was to investigate the effects of AMFJ on exploratory behaviour and locomotor activity in male Wistar rats. AMFJ was administered orally for 7, 14, 21, and 30 days at three increasing doses (2.5, 5, and 10 ml kg−1). The changes in exploratory behaviour and locomotor activity were recorded in an Opto Varimex apparatus. It was found that the low doses of AMFJ (2.5 and 5 ml kg−1) for all treatment periods did not significantly affect exploratory behaviour and locomotor activity of rats compared to the saline-treated controls. AMFJ at the highest dose of 10 ml kg−1 had no significant effect on exploration and locomotion for the treatment periods of 7 and 14 days, while for the periods of 21 and 30 days it significantly decreased the number of horizontal and vertical movements, which might be the result of a sedative effect. At all the doses and testing periods, AMFJ did not disturb the progressive decrease in motor behaviour, suggesting habituation.

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Acta Alimentaria
Authors: N. Topic Popovic, A. Benussi Skukan, P. Dzidara, I. Strunjak-Perovic, S. Kepec, J. Barisic, and R. Coz-Rakovac

Listeria monocytogenes is a bacterium widespread in the environment, which has a capacity to survive and grow under various conditions. The bacterial growth results from interactions when subjected to various temperatures, pH levels, and NaCl concentrations were examined by measurements and predictive modelling. Good correlation across the range of growth conditions was shown among observed and predicted growth values, having similar trends and minimal deflections for pH levels 5.0 and 6.0. The growth condition in the 8% NaCl concentration (pH 7.0, temperature 4 °C) resulted with a growth curve of 1 log interval greater than the fitted curve for all the measurements. In all of the cases, there were consistent increases in the rates and decreases in the lag time when the growth temperature increased. Higher incubation temperatures provided higher growth rates as 30 °C and 35 °C yielded double increase of the fitted rate. Fitted and measured growth rates for salinity conditions were significantly different (P<0.05). Comparison of doubling times showed good compatibility, particularly at lower temperatures. Critical use of a model is suggested, although it may enable microbiologists to limit the need of challenge tests and to make rapid and realistic prediction of the growth of L. monocytogenes under conditions relevant to a range of aquatic and other products examined.

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Patterson, M. F., Quinn, M., Simpson, R. & Gilmour, A. (1995): Sensitivity of vegetative pathogens to high hydrostatic pressure treatment in phosphate-buffered saline and foods. J. Fd Prot. , 58 , 524-529. Sensitivity of

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