Authors:Daria A. Morozova, Vladimir N. Voronin, and Alexey V. Katokhin
in 1% agarose gel containing 0.1% ethidium bromide in Tris-acetate buffer. Before DNA sequencing, the PCR products were purified on spin columns using Sephadex G-50 Superfine sorbent (GE Healthcare, USA). Sanger sequencing was performed using Big Dye
Authors:L. Kredics, Zsuzsanna Antal, A. Szekeres, L. Hatvani, L. Manczinger, Cs. Vágvölgyi, and Erzsébet Nagy
Stepanov, V. M., Rudenskaya, G. N., Gaida, A. V., Osterman, A. L.: Affinity chromatography of proteolytic enzymes on silica-based biospecific sorbents. J Biochem Biophys Methods 5 , 177-186 (1981).
Affinity chromatography of
Authors:N. Almási, Z. Murlasits, A. Al-awar, Á. Csonka, S. Dvorácskó, C. Tömböly, S. Török, D. Bester, A. Pósa, C. Varga, and K. Kupai
for 20 min. The levels of 26S proteasome, ubiquitin, 3-NT, TRPV1 and CGRP were determined by quantitative Enzyme Linked Immuno Sorbent Assay (Delisa and deLateur), using rat marker specific ELISA kits (GenAsia Biotech Co. and SunRed Biotechnology