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Elad, Y., Kapat, A.: The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. Eur J Plant Pathol 105 , 177-189 (1999). The role of Trichoderma harzianum protease in the biocontrol of Botrytis
Trichoderma sp. capable of chestnut blight biocontrol. FEMS Microbiol Lett 126 , 249 (1995). Randomly amplified polymorphic DNA fingerprinting identifies subgroups of Trichoderma viride and other Trichoderma sp. capable of
A total of 36 UV-induced mutants with altered colony morphology were isolated from strain Trichoderma harzianum T334, a potential biocontrol agent against plant pathogenic fungi with the ability to produce constitutively low levels of chitinases. The level of constitutive β -1,4- N -acetyl-glucosaminidase production in standing and shaken cultures under non-inductive conditions was tested in mutants and compared to that of the parental strain. About 30% of the mutants showed significantly increased levels of enzyme production, with strain T334 col26a being the best producer. This mutant and the parental strain were subjected to in vitro confrontation assays with plant pathogenic Fusarium culmorum, Pythium debaryanum and Rhizoctonia solani strains. The mutant derivative could be characterized by significantly higher biocontrol index values than the parental strain in each experiment, suggesting, that mutants with improved constitutive extracellular chitinase secretion could be applied for biocontrol purposes against fungal plant pathogens.
–249. 7 Tamazawa K, Shintani H, Tamazawa Y, et al. Sterilization effect of wet oxygen plasma in the bubbling method. Biocontrol Sci. 2015; 20: 255–261. 8 Shintani H
Bacillus thuringiensis var. kurstaki growing in an economic production medium . Biocontrol Sci Techn 23 , 756 – 767 ( 2013 ). 9. Ghribi , D. , Zouari , N. , Jaoua , S
. , Maeda , T. , Kamei , M. and Kourai , H. ( 2006 ): Pathogenic bacteria carried by companion animals and their susceptibility to antibacterial agents . Biocontrol Sci. 11 , 1 – 9
. : Chestnut blight in Europe: Diversity of Cryphonectria parasitica , hypovirulence and biocontrol . For Snow Landsc Res 76 , 361 – 367 ( 2001 ). 71. Seemann , D. , Bouffier
.: Inhibitory furanone produced by the biocontrol agent Trichoderma harzianum . Phytochemistry 31 , 485-486 (1992). Inhibitory furanone produced by the biocontrol agent Trichoderma harzianum Phytochemistry 31 485 486 Haran, S
The blowfly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) is the primary agent of cutaneous myiasis of sheep in northern Europe, southern Africa, Australia and New Zealand. As the application of chemicals has several disadvantages, alternative control measures of traumatic myiasis of livestock must be developed. In this study, the use of entomopathogenic nematodes (EPNs) as potential biocontrol agents against second instar larvae of Lucilia sericata was considered. The following nematode species were tested: Heterorhabditis bacteriophora (IS 5, HHU 1, Hmo1, HNC 1, HAZ 36, Hbrecon, HHU 2, HAZ 29, HHP 88, HHU 3, HHU 4 and HGua), Steinernema intermedia, NC513 strain of S. glaserii, S. anomali, S. riobrave, Steinernema sp. and 5 strains of S. feltiae (22, Vija Norway, HU 1, scp, and IS 6). None of the examined EPN species or strains showed larvicidal efficacy at 37°C (no killing effect was observed in the case of the two heat-tolerant strains - H. bacteriophora and S. feltiae) against L. sericata larvae. At lower temperatures (20°C and 25°C) only strainsof S. feltiae were found to be active. The overall odds ratios calculated for L. sericata maggots to contract S. feltiae nematode infection show significant (p < 0.05) effect only in the case of strains HU 1, 22 and IS 6. In the case of strains HU 1 and 22 parasitic forms of S. feltiae could be detected in the dead larvae of L. sericata. Strain IS 6 (and also Vija Norway at 20°C) penetrated and killed fly larvae, but only adult forms of the nematode occurred in the cadavers.
416 417 Elad, Y., Kapat, A.: The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea . Eur J Plant Pathol 105 , 177-189 (1999
