Authors:László Fésüs, Attila Zsolnai, István Anton, and László Sáfár
The first results of the Hungarian sheep prion protein (PrP) genotyping programme are discussed in this paper. To obtain initial genotype frequency data 10 commercial (Hungarian Merino, German Mutton Merino, Merino Landschaf, German Blackheaded, Suffolk, Texel, Ile de France, Charollais, Lacaune, British Milksheep) and 4 indigenous (Gyimes Racka, Hortobágy Racka, Tsigaja, Cikta) breeds were sampled in 2003 and 2004, and the PrP genotypes were determined by microsequencing analysis with capillary electrophoresis. In all commercial breeds, a higher number of sheep were genotyped in 2005 (3648) and in 2006 (3834) within the breeding programme to increase scrapie resistance, and the estimated frequency data were compared to the initial figures to evaluate the efficiency of selection. The new developments arising from the identification of the so-called ‘atypical’ scrapie cases are also discussed.
Authors:S. Oueslati, S. Hadj Fredj, R. Belhaj, H. Siala, A. Bibi, and Taieb Messaoud
The analysis of some extra- and intragenic markers within or closely linked to the cystic fibrosis transmembrane regulator (CFTR) gene is useful as a molecular method in clinical linkage analysis. Indeed, knowing that the molecular basis of cystic fibrosis (CF) is highly heterogeneous in our population, the study of haplotype association with normal and CF chromosomes could be very helpful in cases where one or both mutations remain unidentified. In this study, we analysed with PCR-RFLP and capillary electrophoresis some extra (pJ3.11, KM19 and XV2C) and intragenic (IVS8CA, IVS17bTA and IVS17bCA) polymorphic markers in 50 normal and 10 Tunisian patients carrying the rare E1104X mutation in order to determine the haplotype associated with this mutation. For the extragenic markers, 8 haplotypes were identified. The most frequent of them are the 221 and 112 accounting for 80% of total haplotypes. For the intragenic markers, five haplotypes were present on the E1104X chromosomes. One of them 16-31-13 accounted for 50%. To our knowledge, this is the first work to be interested to the haplotypes linked to the E1104X mutation. This preliminary study of haplotypes could be a helpful method to determine the molecular lesions responsible of this pathology.
Ruiz-Martinez MC, Berka J, Belenkii A, et al. DNA sequencing by
capillaryelectrophoresis with replaceable linear polyacrylamide and
laser-induced fluorescence detection. Anal Chem. 1993; 65: 2851
Authors:Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, and Gyula Balka
manually and purified by the use of Qiagen Gel Extraction Kit (Qiagen). Bidirectional Sanger sequencing reaction was performed with the corresponding primers, and capillaryelectrophoresis was made by a commercial provider (Hungarian Natural History Museum
Authors:György Szabó, László Környei, Éva Keller, Gabriella Lengyel, and János Fehér
risk of driving under the influence of alcohol when determined by capillaryelectrophoresis Forensic Sci. Int. 170 175 – 178 .
. J. Fehér G. Lengyel G. Szabó 2006 Carbohydrate-deficient transferrin as the marker of alcohol