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Habib, I., Sampers, I., Uyttendaele, M., Berkvens, D., De Zutter, L.: Performance characteristics and estimation of measurement uncertainty of three plating procedures for Campylobacter enumeration in chicken meat. Food Microbiol. 25 (1), 65–74 (2008

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The aim of this study was to compare an enzyme-linked fluorescent assay (ELFA)-based and two real-time polymerase chain reaction (PCR) methods with the results of the standard culture-based method EN ISO 6579:2002 (bacteriological standard method used in the European Union) for the detection of Salmonella spp. in raw chicken meat. Our investigations were performed on 141 poultry samples sorted from supermarkets.Relative accuracy, relative specificity and relative sensitivity were determined. According to the ISO 16140:2003 criteria for validation of alternative microbiological methods, the ELFA-based method (VIDAS ICS2 + SLM), and real-time PCR methods (TaqMan, Bax) were comparable to the reference standard method for the detection of Salmonella spp. in chicken meat. The use of these methods provide results within 48 hours with high sensitivity (100%). The TaqMan real-time PCR showed a relative specificity of 98% and both of the real-time PCR methods presented 100%.The VIDAS ICS2 + SLM and the Bax real-time PCR methods showed the highest relative accuracy (100%) and 99% in case of the TaqMan method. In conclusion, both the real-time PCR and the ELFA-based assay can be used as a rapid and user-friendly diagnostic method for detection of Salmonella spp. in chicken meat samples.

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Acta Veterinaria Hungarica
Authors: Orsolya Erdősi, Katalin Szakmár, Zsuzsanna Szili, Géza Szita, Sándor Bernáth, József Sövényi, and Péter Laczay

. Churruca , E. , Girbau , C. , Martínez , I. , Mateo , E. , Alonso , R. and Fernández-Astorga , A. ( 2007 ): Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real- time nucleic acid sequence

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Acta Veterinaria Hungarica
Authors: Tulay Elal Mus, Figen Cetinkaya, Recep Cibik, Gul Ece Soyutemiz, Husniye Simsek, and Nilay Coplu

. Choi , J. M. and Woo , G. J. ( 2013 ): Molecular characterization of high-level gentamicin-resistant Enterococcus faecalis from chicken meat in Korea . Int. J. Food Microbiol. 165 , 1 – 6 . CLSI (Clinical

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chicken meats ( Leinweber et al., 2018 ). The presence of VREfm isolates carrying the van A gene in poultry and environmental samples poses a significant risk to humans, animals and the environment, as vancomycin resistance can be transferred to other

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Acta Veterinaria Hungarica
Authors: Benjámin Kövesi, Szabina Kulcsár, Mátyás Cserháti, Márta Erdélyi, Zsolt Ancsin, Erika Zándoki, Miklós Mézes, and Krisztián Balogh

. Yamauchi , K. , Manabe , N. , Matsumoto , Y. , Takenoyama , S. I. and Yamauchi , K. E. ( 2014 ): Increased collagen III in culled chicken meat after feeding dietary wood charcoal and vinegar contributes to palatability and tenderness . Anim

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Acta Veterinaria Hungarica
Authors: Orsolya Erdősi, Katalin Szakmár, Olivér Reichart, Zsuzsanna Szili, Noémi László, Péter Székely Körmöczy, and Péter Laczay

of Listeria monocytogenes by real-time PCR in retail ground chicken meat. Foodborne Pathog. Dis. 3 , 347–354. Martinez-Ruarez J. V. Evaluation of effects of primary and secondary

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Zorman, T., Heyndrickx, M., Uzunovic-Kamberovic, S. and Smole Mozina, S. (2006): Genotyping of Campylobacter coli and C. jejuni from retail chicken meat and humans with campylobacteriosis in Slovenia and Bosnia and Herzegovina. Int. J. Food

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314 Huang YX, Miller EL: Iron-induced TBARS as an indicator of oxidative stability of fresh chicken meat. Proc. 11th European Symp. on the Quality of Poultry Meat, Tours, Vol. 1, pp. 430

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, B., Nagai, A., Kozawa, K., Nakajima, T., Mikami, T. and Kimura, H. (2004): Isolation and phylogenetic analysis of Arcobacter spp. in ground chicken meat and environmental water in Japan and Thailand. Microbiol. Immunol. 48 , 527

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