Authors:L.V. Shchukina, T.A. Pshenichnikova, E.K. Khlestkina, S. Misheva, T. Kartseva, A. Abugalieva, and A. Börner
Bread wheat is the primary bread crop in the majority of countries in the world. The most important product that is manufactured from its grain and flour is yeast bread. In order to obtain an excellent bread, grain with high physical properties is needed for flour and dough. The Russian spring wheat cultivar Saratovskaya 29 is characterized by its exclusively high physical properties of flour and dough. The purpose of this work was to identify the chromosomes carrying the main loci for these traits in Saratovskaya 29 and to map them using recombinant substitution lines genotyped with molecular markers. A set of inter-varietal substitution lines Saratovskaya 29 (Yanetzkis Probat) was used to identify the “critical” chromosomes. The donor of individual chromosomes is a spring cultivar with average dough strength and tenacity. Substitution of 1D and 4D*7A chromosomes in the genetic background of Saratovskaya 29 resulted in a significant decrease in the physical properties of the dough. Such a deterioration in the case of 1D chromosome might be related to the variability of gluten protein composition. With the help of recombinant substitution double haploid lines obtained from a Saratovskaya 29 (Yanetzkis Probat 4D*7A) substitution line the region on the 4D chromosome was revealed in the strong-flour cultivar Saratovskaya 29, with the microsatellite locus Xgwm0165 to be associated with the unique physical properties of flour and dough. The detected locus is not related to the composition gluten proteins. These locus may be recommended to breeders for the selection of strong-flour cultivars. Additionally, a QTL associated with vitreousness of grain was mapped in the short arm of chromosome 7A.
Authors:A. F. Bálint, G. Kovács, A. Börner, G. Galiba, and J. Sutka
Niemeyer, H. M., Jerez, J. M. (1997): Chromosomallocation of genes for hydroxamic acid accumulation in Triticum aestivum L. (wheat) using wheat aneuploids and wheat substitution lines. Heredity , 79 , 10-14.
Authors:B.W. Campbell, Y. Liu, K. Wise, Y. Jin, and H.W. Ohm
Stem rust caused by Puccinia graminis f. sp tritici of wheat (Triticum aestivum L.) is one of the most destructive cereal diseases globally. Concern about the disease has increased since 1999 with the discovery in Uganda of a new virulent race of Pgt, designated as race TTKSK (also known as Ug99). The objectives of this experiment were to characterize the resistance and to determine the chromosomal location of the stem rust resistance in the spring wheat line PI 410966. A mapping population was developed from a cross between PI 410966 and a susceptible wheat line OK3040. An inoculation test with isolate 04KEN156/04 of race TTKSK was conducted at the USDA-ARS Cereal Disease Laboratory in the F6:7 generation, and the F6:7 phenotypic data were used to genetically map the resistance gene to the centromeric region on chromosome 2BS. The single locus explained the observed F6:7 resistant and susceptible scores. The location of the gene and molecular marker banding profiles of the diagnostic markers suggest that the stem rust resistance gene in PI 410966 could be a new gene, an allele of Sr36, or Sr36.
Authors:I. Karsai, P. Szűcs, K. Mészáros, K. Puskás, Z. Bedő, and O. Veisz
A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.