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Report Series (Rare Diseases Collection): Prevalence of rare diseases: Bibliogaphic data. Number 2. 2009. Roll, K.: The influence of regional health care structures on delay in diagnosis of rare
Bigot, K., Mignon-Grasteau, S., Picard, M. and Tesseraud, S. (2003): Effects of delayed feed intake on body, intestine, and muscle development in neonate broilers. Poult. Sci. 82 , 781
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119 129 Pack, G. T., Gallo, S. J.: The culpability for delay in treatment of cancer. Am. J. Cancer, 1938, 33 , 443
Purpose: The clinical demand for bone grafting materials necessitated the development of animal models. Critical size defect model has been criticized recently, mainly for its inaccuracy. Our objective was to develop a dependable animal model that would provide compromised bone healing, and would allow the investigation of bone substitutes. Methods: In the first group a critical size defect was created in the femur of adult male Wistar rats, and a non-critical defect in the remaining animals (Groups II, III and IV). The defect was left empty in group II, while in groups III and IV a spacer was interposed into the gap. Osteoblast activity was evaluated by NanoSPECT/CT imaging system. New bone formation and assessment of a union or non-union was observed by μCT and histology. Results: The interposition model proved to be highly reproducible and provided a bone defect with compromised bone healing. Significant bone regeneration processes were observed four weeks after removal of the spacer. Conclusion: Our results have shown that when early bone healing is inhibited by the physical interposition of a spacer, the regeneration process is compromised for a further 4 weeks and results in a bone defect during the time-course of the study.
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