fingerprinting is playing an increasingly important role in the quality control of TCM, and has proven to be practical and scientific [ 9 ]. Recently, the chromatographic fingerprint technique has been introduced as a tool to evaluate the quality of herbal
cultivars of M. oleifera using HPLC with photodiode array detection and electrospray ionization–MS (HPLC–PDA–ESI–MS) analysis [ 20 ]. Fingerprinting techniques can provide an overall profile of almost every component of medicinal plants and are
Traditional Chinese Medicine with Chromatographic Fingerprint, Guangzhou, 2001, pp. 18–35. Y.X. Zhou, H.M. Lei, Y.H. Xu, L.X. Wei , and X.F. Xu , Research Technology of Fingerprint of Chinese
://www.who.int (accessed February 22, 2010). [18]. Drug Administration Bureau of China, Requirements for Studying Fingerprints of Traditional Chinese Medicine Injection , Drug Administration
Heun M, Schäfer-Pregl R, Klawan D, Castagna R, Accerbi M, Borghi B, Salamini F (1997) Site of Einkorn Wheat Domestication Identified by DNA Fingerprinting. Science 278: 1312–1314. Salamini F
chemometric tools to identify the geographical origin of yellow wine based on the fingerprint of volatile organic compounds. In this article, an approach was developed based on a color mixing method to provide visual color differences between different 2D
strategy was limited because Q-TOF and Qtrap-MS have not been widely equipped in many pharmaceutical factories in China. In the present study, the commonly equipped diode array detector (DAD) was initially applied for the quantitative fingerprint analysis
A high-performance thin-layer chromatography (HPTLC) method was developed for simple and rapid chemical analyses of multi-ingredient botanicals. The method was based on comparison of the fingerprint of the marketed multi-ingredient botanical with the fingerprints of the extracts of its component plants and of a handmade mixture of the constituent extracts in a ratio as similar as possible to that of the market product, when labeled. Planar chromatography analysis was completed by a densitometric scansion. The analyses revealed a good correspondence between the market products and the handmade mixture of standard extracts.
Summary
The potential of high-performance thin-layer chromatographic (HPTLC) fingerprinting in identifying differences in sandalwood essential oils from 5 sandalwood species, namely, Santalum album, Santalum spicatum, Santalum austrocaledonicum, Santalum panic-ulatum, Santalum lanceolatum, and a natural substitute for sandalwood, Osyris lanceolata, was explored. Variation was observed in the profile of bands (R F values and color) and peak intensity profiles displayed by the essential oils across and within the essential oils studied with some bands being unique to the individual species. The potential of HPTLC fingerprinting as a quality control tool in authenticating sandalwood oils in the sandalwood industry was demonstrated in the present study.
In the 5th European Pharmacopoeia a thin-layer chromatographic (TLC) technique based on analysis of ascorbic acid only is stipulated for qualitative analysis of Rosae pseudofructus, a drug from the dog rose. In this paper a new, simple method is proposed in which the TLC pattern of the flavonoid content is used for effective and reliable quality control of the drug. The method can also be used for identification of different Rosa L. species. The proposed assay uses ultrasound-assisted extraction then clean-up by solid-phase extraction before chromatographic analysis. Quercetin 3-rhamnoside, quercetin 3-glucoside, quercetin 3-rutinoside, catechin, and gallic acid are used as markers. The results reveal that the polyphenol composition of rose pseudo-fruits furnishes a specific TLC chromatogram fingerprint which might be helpful for quality assurance and detection of adulteration of crude extracts.
