Authors:Volker Micheel, Benedikt Hogan, Rivo Rakotoarivelo, Raphael Rakotozandrindrainy, Fetra Razafimanatsoa, Tsiriniaina Razafindrabe, Jean Rakotondrainiarivelo, Sabine Crusius, Sven Poppert, Norbert Schwarz, Jürgen May, Hagen Frickmann, and Ralf Hagen
This study assesses the nasal occurrence of β-lactamase-producing Enterobacteriaceae both in patients in a hospital department of infectious diseases at admission and in healthy Madagascan students and health care workers.Nasal swabs from 681 students, 824 health care workers, and 169 patients were obtained in Antananarivo, Madagascar, and transferred to Germany. Screening for β-lactamase (ESBL, ampC) producing Enterobacteriaceae was performed by cultural and molecular approaches, comprising Brilliance ESBL agar, E-testing, ABCD-testing, and commercial hyplex ESBL and SuperBug ID PCR.Regarding ESBL-positive strains and strains with resistance against at least three out of the four tested bactericidal antibiotic drugs, 0.3% (five out of 1541) of the students and health care workers group showed nasal colonization, whereas colonization was observed in 7.1% (12 out of 169) of the hospitalized patients at admission. No appreciably reduced detection rates after sample storage and intercontinental transport were observed.A considerable proportion of nasal colonization with cephalosporin-resistant Enterobacteriaceae was demonstrated in Madagascan hospital patients at admission, posing a risk of developing future endogenous infections. The nasal colonization of healthy individuals was negligible. Good storage and transport stability of Enterobacteriaceae will allow for future studies even in areas difficult to access.
Authors:C. Çirak, A. K. Ayan, K. Kevseroglu, and T. Özen
Hypericum perforatum L. (St. John's wort) is a
well-known traditional medicinal plant that has been used for centuries for the
treatment of several diseases and this feature is obviously attributed to the
presence of a broad spectrum of secondary metabolites including hypericins. In
the present study, the aim was to determine total hypericin content of this
plant from wild population of Northern Turkey. Thus, Hypericum perforatum
plants representing a total of 45 wild populations were collected from 15 sites
in each of 3 regions, during flowering and subsequently assayed for total
hypericin. Hypericin content in these wild populations varied from 0.28 to 4.46
mg/g dry matter. According to the results of analysis of variance, there was a
significant difference in hypericin content between sites as well as regions.
One of the problems associated with medicinal plant preparations is the extreme
variability in the phytomedicinal and marker compounds. Products containing H.
perforatum have varied greatly in content of secondary metabolites since it is
currently supplied by cultivated and wild-harvested materials. Therefore the
variation of phytomedicinals like hypericin in H. perforatum, especially from
wild populations has a great importance in term of health care and herbal
Authors:Ralf Matthias Hagen, Hagen Hinz, and Hagen Frickmann
, Rakotozandrindrainy R , Razafimanatsoa F , Razafindrabe T , Rakotondrainiarivelo JP , Crusius S , Poppert S , Schwarz NG , May J , Frickmann H , Hagen RM : Identification of nasal colonization with ß-lactamase-producing Enterobacteriaceae in patients, health
Authors:Julia Münch, Ralf Matthias Hagen, Martin Müller, Viktor Kellert, Dorothea Franziska Wiemer, Rebecca Hinz, Norbert Georg Schwarz, and Hagen Frickmann
, Razafindrabe T , Rakotondrainiarivelo JP , Crusius S , Poppert S , Schwarz NG , May J , Frickmann H , Hagen RM : Identification of nasal colonization with ß-lactamase-producing Enterobacteriaceae in patients, healthcare workers and students in