and deteriorate rapidly with the high susceptibility to fungal infections by a broad range of bacterial phytopathogens, resulting in severe economic losses. Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill. is a representative necrotrophic fungal
Authors:V. Parrag, J. Felföldi, L. Baranyai, A. Geösel, and F. Firtha
From the nineteen-nineties, cobweb disease caused serious losses for the mushroom sector in Europe, in the USA, and in Australia (Fletcher & Gaze, 2008), so it is one of the most notable fungal infections of cultivated white button mushroom (Agaricus bisporus). The aim of this study was to identify cobweb disease (Cladobortyum dendroides) caused cap spotting and brownish rot on the mushroom sporocarp, and to find a proper discrimination method in the case of this infection.Fruiting body samples were divided into 4 groups, a control one and three others treated with different chemicals that are tested against fungal infections. The groups were subdivided into 2 portions and the first was infected with cobweb disease. Images of the caps were recorded and their hyperspectral images were acquired in the wavelength range of 900–1700 nm.On the hyperspectral images infected and healthy areas were selected, on these average spectra differences were found around the known water peaks (1200 and 1450 nm). The spatial distribution of the water content can be used for the detection of the spoilage, because the infected areas showed different reflection values at these water absorption peaks.Support Vector Machine method was applied successfully to discriminate between the infected and control groups and Monte Carlo cross-validation was carried out.
Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can be used as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features for example fast growing and resistance against the disease and climatic fluctuations, etc. Since Sida is in the beginning phase of domestication, it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent, the need of seed showing of driller should tenfold 200 thousand seeds/acre instead of 10–20 thousand, which is not available and expensive. Therefore the practical purpose of our research of seed physiology was to increase the seed germination percent in the available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seeds. However, in our germination tests, utilizing the scarified seeds with hot water (65, 80 and 95 °C), from 29.3% to 46% germinated from those samples, which were collected from the population of S. hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When applying physically scarified use, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that there is a close relationship between the collecting time of the seeds and the ration of seed infections, as well as germination percentage. Thus, the 2009 season was the most favourable in case of contamination (control: 17.3% and 80 °C treatment: 0%) as well as germination percent. It could be concluded that the best season for our findings was 2009 due to autumn harvest of Sida seeds. In our opinion, the autumn harvesting should be the best time to overcome the problem of the low germination and high infection percentage. We also discovered that apparently there is a close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognitions, we modified our technique in such a way that we fractionated the seeds based on their fresh weight / or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore by this special priming process, we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 °C without illumination).
( 2013 ), Relation between the germination and infection ratio on Sida hermaphrodita L Rusby seeds under hotwater treatment . International Journal of Horticulture Sciences , 19 ( 1 – 2 ), 117
, D. , Hutchinson , J. , Kelly , S. , … & the Canadian Nosocomial Infection Surveillance Program ( 2010 ): Hypervirulent Clostridium difficile strains in hospitalized patients, Canada . Emerg. Infect. Dis. , 16 , 678 – 681