Search Results

You are looking at 1 - 4 of 4 items for :

  • "lamotrigine" x
  • Chemistry and Chemical Engineering x
  • Refine by Access: All Content x
Clear All

An HPTLC method with densitometric detection at 280 nm has been established for quantification of lamotrigine in human serum. Liquid-liquid extraction with ethyl acetate, with chloramphenicol as internal standard, was followed by chromatography on silica gel F254 plates with ethyl acetate-methanol-32% aqueous ammonia 17:2:1 (v/v) as mobile phase. The method was validated for linearity, precision, and accuracy. Calibration plots were linear in the range 0.6 to 300 ng per band, corresponding to 0.06–30.00 ng μL−1 lamotrigine in human serum after extraction and application of 1 μL to the chromatographic plate. The correlation coefficient was 0.998. Intra-assay and inter-assay precision, as relative standard deviation (RSD), were in the range 0.53–2.91% (n = 3) and 1.58–2.98% (n = 9), respectively. The limits of detection and quantification were 0.016 and 0.042 ng, respectively. Accuracy, calculated as percentage recovery, was between 94.09 and 101.30%, with RSD no higher than 3.52%. The method was selective for both lamotrigine and the internal standard. The method enables rapid, precise, accurate, selective, and sensitive quantitative analysis of lamotrigine in human serum.

Restricted access

), whereas formic acid and lamotrigine (≥98%), as the internal standard (IS), were obtained from Sigma-Aldrich (St. Louis, MO, USA). Blank human plasma in ethylenediaminetetraacetic acid-tri-potassium anticoagulant (mixed gender) was from Biochemed Services

Open access

. Moisture-dependent crystallization of amorphous lamotrigine mesylate . J Pharm Sci . 1996 ; 85 : 1215 – 1219 . 10.1021/js9600484 . 32. Tong , P , Zografi , G . Solid

Restricted access

Pharmaceuticals PLC (Budapest, Hungary); quetiapine, Adamed Sp. z o.o. (Pieńków, Poland); levomepromazine, PROTERAPIA Sp. z o.o. (Warsaw, Poland); lamotrigine, Glenmark Pharmaceuticals (Prague, Czech Republic); moclobemide, Hoffmann LaRoche (Basel, Switzerland

Open access