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, E., Ganal, M.W., Röder, M.S. 2000b. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43 :689–697. Röder M.S. Isolation and

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92 1073 1077 Korzun, V., A. Bömer, A.J. Worland, C.N. Law and M.S. Röder, 1997: Application of microsatellite markers to distinguish intervarietal

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microsatellite markers. Theor. Appl. Genet. 105 :699–707. Ganal M.W. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

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Plaschke J., Ganal M.W., Röder M.S. (1995): Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91:1001–1007. Röder M

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Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding 2 :225

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., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding 2 :225–238. Rafalski A

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Cereal Research Communications
Authors: F. Akfirat, Y. Aydin, F. Ertugrul, S. Hasancebi, H. Budak, K. Akan, Z. Mert, N. Bolat, and A. Uncuoglu

microsatellite markers linked to a powdery mildew resistance gene in wheat line “Lankao 90(6)”. Plant Breeding 127 :346–349. Shen T.M. Chromosome location and microsatellite markers linked

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Acta Biologica Hungarica
Authors: Petra Zenke, Á. Maróti-Agóts, Zs. Pádár, and L. Zöldág

Demand for correct and cost-effective genetic-based identification and parentage control has increasing importance in domestic animals, including dogs. In our study the applicability of a canine hyperpolymorphic microsatellite marker — which localized in the WILMS-TF (tumor factor) gene — was examined in mixed breed and purebred canine populations. The redesigned and shortened amplicons were genotyped using an allelic ladder which was constructed from sequence verified fragments. The nomenclature for allele calling based on repetition structures is suitable for international comparisons. Our study justified the potential use and efficiency of the marker D18S12 in parentage control.

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5937 5943 Huang, X.Q., Börner, A., Röder, M.S., Ganal, M.W. 2002. Assessing genetic diversity of wheat ( Triticum aestivum L.) germplasm using microsatellite markers. Theor

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Grain protein content (GPC) in durum wheat is a crucial determinant of pasta quality and as such is an important economic factor. This study was carried out to determine the microsatellite markers (SSRs) as associated with GPC in durum wheat grown under normal and moisture stress conditions. F3 and F4 population derived from 151 F2 individuals developed from a cross between Oste-Gata (drought tolerant) and Massara-1 (drought susceptible) genotypes, were used. The population was evaluated under four environmental conditions (two irrigation regimes in two growing seasons). The results of single marker regression analysis (SMA) revealed that 2, 4 and 10 markers to be associated with GPC, test weight (TW) and 1000 grain weight (TGW), respectively. These markers explained between 4.4 and 21.8% of the phenotypic variation in either environmental condition. The most significant marker observed for GPC was located on 5B chromosome near Xgwm408 under normal conditions and the other marker was observed on 1A, explaining about 15% of phenotypic variance. However, it was not recognized any marker related to GPC under drought stress conditions. Xgwm408 marker was coincident with the markers identified for TW, TGW and components of grain yield under drought stress conditions. In spite of 5B, the other chromosomes such as 2B and 3B were related to quantitative traits like TW and TGW. Composite interval mapping (CIM) identified 4 and 5 putative minor and major QTL for TW and TGW, respectively. Two QTL near Xbarc101 and Xbarc124 markers on 3B and 2B chromosome, explained up to 45.2 and 6% of phenotypic variations of TGW and TW, respectively.

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