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successively extracted with n -hexane, chloroform, ethyl acetate, and finally with water. The chloroform soluble fraction was subjected to normal phase column chromatography using silica gel 60 (0.062–0.200 mm; Merch) resulting in the isolation of three
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with the mass selective detector of an Agilent HP 5973 mass spectrometer operating in electron ionization mode. A DB-5 fused silica capillary column (30 m x 0.25 mm, film thickness 0.25 μm) was used, and the chromatography conditions were as follows
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