Authors:M.D. Boatwright, A.K. Fritz, and D.L. Wetzel
. A new solution for an old problem: the regiochemical distribution of the acyl chains in galactolipids can be established by electrospray ionization tandemmassspectrometry Rapid Commun . Mass Spectrom. 17 : 1982 – 1994
Authors:X. Luo, C. Han, X. Deng, D. Zhu, Y. Liu, and Y. Yan
Seed germination is a new beginning for the crop life cycle, which is closely related to seed sprouting and subsequent plant growth and development, and ultimately affects grain yield and quality. Salt stress is one of the most important abiotic stress factors that restrict crop production. Therefore, it is highly important to improve crop salt tolerance and sufficient utilization of saline-alkali land. In this study, we identified the phosphorylated proteins involved in salt stress response by combining SEM, 2-DE, Pro-Q Diamond staining and tandem mass spectrometry. The results showed that salt stress significantly inhibited seed germination and starch degradation. In total, 14 phosphorylated protein spots (11 unique proteins) in the embryo and 6 phosphorylated protein spots (4 unique proteins) in the endosperm were identified, which mainly involved in stress/defense, protein metabolism and energy metabolism. The phosphorylation of some proteins such as cold regulated proteins, 27K protein, EF-1β and superoxide dismutase could play important roles in salt stress tolerance.
Authors:Y. Liu, S. Wang, C. Wang, G. Chen, H. Cao, Y. Wang, W. Ma, Y. Hu, and Y. Yan
A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1Sl/1B substitution line CS-1Sl(1B) was carried out in the current study. A total of 78 differentially expressed protein (DEP) spots with at least 2-fold expression difference were detected by two-dimensional electrophoresis (2-DE). Among these, 73 protein spots representing 55 differentially expressed proteins (DEPs) were successfully identified by matrix-assisted laser desorption/ionization time-offlight tandem mass spectrometry (MALDI-TOF/TOF-MS). Differential protein spots between the two genotypes were analyzed by cluster software, which revealed significant proteome differences. There were 39 common spots (including 33 DEPs) that showed significant difference between the two lines across five grain developmental stages, of which 14 DEP spots (including 11 DEPs) were mainly involved in carbohydrate metabolism that were encoded by the genes on 1B chromosome while 25 DEP spots (including 12 DEPs) were mainly related to stress response and gluten quality that were encoded by 1S1 chromosome. These results indicated that the Sl genome harbors more stress and quality related genes that are potential valuable for improving wheat stress resistance and product quality.
Authors:Markus Krohn, Thomas Wanek, Marie-Claude Menet, Andreas Noack, Xavier Declèves, Oliver Langer, Wolfgang Löscher, and Jens Pahnke
acid (3 μL at 99%, v/v). The stable isotope-labeled peptide mixture (750 fmol of each labeled peptide) was added in tryptic digest before ultrahigh-performance liquid chromatography–tandemmassspectrometry (UHPLC–MS/MS) analysis
Authors:Matthias F. Emele, Matti Karg, Helmut Hotzel, Linda Graaf-van Bloois, Uwe Groß, Oliver Bader, and Andreas E. Zautner
, Baxter DJ , Cannon WR , Kalyanaraman A , Kulkarni G , Callister SJ . Proteotyping of microbial communities by optimization of tandemmassspectrometry data interpretation . Pac Symp Biocomput Pac Symp