Authors:L. Kóbori, T. Németh, B. Nemes, G. Dallos, P. Sótonyi Jr., I. Fehérvári, A. Patonai, M. J. H. Slooff, J. Járay and K. P. De Jong
Hepatic artery thrombosis is a major cause of graft failure in liver transplantation. Use of donor interponates are common, but results are controversial because of necrosis or thrombosis after rejection. Reperfusion injury, hypoxia and free radical production determinate the survival. The aim of the study was to create an 'ideal' arterial interponate. Autologous, tubular graft lined with mesothelial cells, prepared from the posterior rectus fascia sheath, was used for iliac artery replacement in eight mongrel dogs for six months under immunosuppression. Patency rate was followed by Doppler ultrasound. Eight grafts remained patent and another two are patent after one year. The patency rate was good (median Doppler flow: 370 cm/sec) and there was no necrosis, thrombosis or aneurysmatic formation. The grafts showed viable morphology with neoangiogenesis, appearance of elastin, smooth muscle and endothelial cells. Electron microscopy showed intact mitochondrial structures without signs of hypoxia. Tissue oxygenation was good in all cases with normal (< 30 ng/ml) myeloperoxidase production. In conclusion, this autologous graft presents good long-term patency rate. Viability, arterialisation and low thrombogenicity are prognostic factors indicating usability of the graft in the clinical practice without the risk of rejection. Further investigations such as cell cultures and standardisation are necessary.
Authors:Péter Csathó, E. Osztoics, J. Csillag, T. Lengyel, L. Gonda, L. Radimszky, G. Baczó, M. Magyar, K. R. Végh, M. Karátsonyi, T. Takács, A. Lukács and T. Németh
Depending on their origin, sedimentary phosphate
rocks (PRs) may differ in their P solubility, and, as a consequence, in their
agronomic effectiveness. The effect of six phosphate rocks (PR) - originating
from Algeria (ALG), North Florida (FLO), North Carolina (NCA), Senegal (SEN)
Morocco (MOR) and Hyperphosphate (HYP) with various P solubility (evaluated by
2% formic acid, 2% citric acid, and neutral ammonium citrate) - as well as
single superphosphate (SSP) and superphosphate + lime (SSP + Ca) (each P source
on 4 P levels, with doses of 0, 100, 400 and 1600 mg P
soil) on the shoot yield of tillering stage spring barley, soil available
P (i.e. H
O, Olsen, Bray1, Lakanen-Erviö (LE) and ammonium lactate
(AL) extractable P contents) were studied in pot experiments set up with acidic
sandy soil (Nyírlugos, Hungary) and acidic clay loam soil (Ragály, Hungary),
both with low P supplies. The average
spring barley shoot yield at the beginning of shooting was 95% higher on the
colloid-rich acidic (pH
: 4.5) clay loam soil than on the
colloid-poor acidic (pH
: 3.8) sandy soil. The differences in the
solubility of phosphate rocks showed close correlation to the differences in P
responses. On both soils, the correlation between total PR-P added and P
responses in spring barley shoot yield was much weaker than that between
neutral ammonium citrate soluble PR-P added and P responses in spring barley
shoot yield. When phosphate rocks were applied as P sources, the comparison of
soil test P methods showed a different picture on the two soils. In the case of
the acidic sandy soil (Nyírlugos), the strongly acid LE-P (r² = 0.83) and
AL-P (r² =0.74) tests gave the highest correlation coefficients with
spring barley responses to P, while on the acidic clay loam soil (Ragály) these
were achieved by the Olsen-P (r² = 0.88) and Bray1-P (r² =0.88)
Authors:L. Kóbori, G. Dallos, Anette S. H. Gouw, Tamás Németh, B. Nemes, I. Fehérvári, A. M. Tegzess, M. J. H. Slooff, K. P. De Jong and F. Perner
Vascular complications in liver transplantation are a major cause of graft failure and mortality. The aim of the study was to create autologous vascular graft without risk of rejection. Posterior rectus fascia sheath lined with peritoneum was used for iliac artery replacement in seven mongrel dogs. The patency was followed by palpation and Doppler ultrasound. The grafts were removed after one month. Five grafts remained patent. The Doppler showed good, relatively increased flow (median flow rate: 383 cm/sec) after one month in all of the cases. Slight increase in diameter was present in all cases. By microscopy the five patent grafts showed viable morphology, fibroblasts, smooth muscle cells and thin fibrin layer in the wall. The grafts were lined partially with a neoendothelial monolayer and a thin fibrin layer. In conclusion, this graft presents an acceptable patency rate and low thrombogenicity, and could be useful in transplantation. Further investigations are needed to study the effect of immunosuppression and rejection on long-term morphology and patency of the grafts.
Authors:K. Szakszon, Z. L. Veres, M. Boros, S. Sz. Kiss, B. Nagy, E. Bálega, á. Papp, E. Németh, I. Pataki and T. Szabó
We report a case of an infant with spontaneous chylothorax due to the congenital malformation of a small lymph vessel of the chest wall. Conservative therapy with omitting long-chain fatty acids from the diet, fat-free nutrition, total parenteral nutrition and intravenous somatostatin did not result in the decrease of pleural effusion. Thoracic surgical intervention performing thoracic duct ligation and using fibrin sealants was applied after 10 days of unsuccessful conservative therapy, and resulted in the complete recovery of the patient. Our experience support the already existing observations, that in cases where the daily loss of chyle exceeds 100 ml per age years and/or lasts longer than 2 weeks, early surgical intervention is recommended.
Authors:Z. Németh, Z. Homonnay, F. Árva, Z. Klencsár, E. Kuzmann, J. Hakl, K. Vad, S. Mészáros, K. Kellner, G. Gritzner and A. Vértes
In this paper we present 57Co emission Mössbauer and AC magnetic susceptibility studies of La0.8Sr0.2CoO3-δ perovskite. The observed coexistence of paramagnetic and magnetic subspectra in the 57Co emission Mössbauer spectra, as well as the difference of their isomer shifts support the existence of electronic phase
separation in this perovskite, in good agreement with the double exchange based cluster model.
Authors:Szilvia Veszelka, Judit Laszy, Tamás Pázmány, László Németh, Izabella Obál, László Fábián, Gábor Szabó, Csongor S. Ábrahám, Mária A. Deli and Zoltán Urbányi
Serum amyloid P component (SAP), a member of the innate immune system, does not penetrate the brain in physiological conditions; however, SAP is a stabilizing component of the amyloid plaques in neurodegenerative diseases. We investigated the cerebrovascular transport of human SAP in animal experiments and in culture blood-brain barrier (BBB) models. After intravenous injection, no SAP could be detected by immunohistochemistry or ELISA in healthy rat brains. Salmonella typhimurium lipopolysaccharide injection increased BBB permeability for SAP and the number of cerebral vessels labeled with fluorescein isothiocyanate (FITC)-SAP in mice. Furthermore, when SAP was injected to the rat hippocampus, a time-dependent decrease in brain concentration was seen demonstrating a rapid SAP efflux transport in vivo. A temperature-dependent bidirectional transport of FITC-SAP was observed in rat brain endothelial monolayers. The permeability coefficient for FITC-SAP was significantly higher in abluminal to luminal (brain to blood) than in the opposite direction. The luminal release of FITC-SAP from loaded endothelial cells was also significantly higher than the abluminal one. Our data indicate the presence of BBB efflux transport mechanisms protecting the brain from SAP penetration. Damaged BBB integrity due to pathological insults may increase brain SAP concentration contributing to development of neurodegenerative diseases.
Authors:E. Hartmann, A. Németh, Gy. Juharosi, Zs. Lénárd, P. Á. Deák, V. Kozma, P. Nagy, Zs. Gerlei, I. Fehérvári, B. Nemes, D. Görög, J. Fazakas, L. Kóbori and A. Doros
Hepatocellular carcinoma, which has developed in liver cirrhosis is a disease where liver transplantation can provide a cure both for the tumour and the underlying liver damage. However, patients can only be transplanted when the tumour number and size do not exceed the Milan criteria. Tumour ablation methods — such as radiofrequency ablation — can provide a chance to make the patient eligible for transplantation. Among the 416 Hungarian liver transplanted patients there are 6 who had received different types of ablative therapy as bridging therapy in different institutions. On the basis of analysis of the patients' data we created a guideline for the treatment of cirrhotic patients with hepatocellular carcinoma with the aim of developing a uniform Hungarian approach.
Authors:E. Horvath-Szanics, J. Perjéssy, A. Klupács, K. Takács, A. Nagy, E. Koppány-Szabó, F. Hegyi, E. Németh-Szerdahelyi, M.Y. Du, Z.R. Wang, J.Q. Kan and Zs. Zalán
The increasing consumer demand for less processed and more natural food products – while improving those products’ quality, safety, and shelf-life – has raised the necessity of chemical preservative replacement. Biopreservation refers to extended storage life and enhanced safety of foods using the natural microflora and (or) their antibacterial products. Chitinolytic enzymes are of biotechnological interest, since their substrate, chitin, is a major structural component of the cell wall of fungi, which are the main cause of the spoilage of food and raw plant material. Among the several organisms, many bacteria produce chitinolytic enzymes, however, this behaviour is not general. The chitinase activity of the lactic acid bacteria is scarcely known and studied.
The aim of the present study was to select Lactobacillus strains that have genes encoding chitinase, furthermore, to detect expressed enzymes and to characterise their chitinase activity. Taking into consideration the importance of chitin-bindig proteins (CBPs) in the chitinase activity, CBPs were also examined. Five Lactobacillus strains out of 43 strains from 12 different species were selected by their chitinase coding gene. The presence of the chitinase and chitin-biding protein production were confirmed, however, no chitinolytic activity has been identified.
Authors:T. Tóth, T. Németh, A. Bidló, F. Dér, M. Fekete, T. Fábián, Z. Gaál, B. Heil, T. Hermann, E. Horváth, G. Kovács, A. Makó, F. Máté, K. Mészáros, Z. Patocskai, F. Speiser, I. Szűcs, G. Tóth, Gy. Várallyay, J. Vass and Sz. Vinogradov