Search Results

You are looking at 1 - 2 of 2 items for

  • Author or Editor: Nikhil Jirankalgikar x
  • All content x
Clear All Modify Search

Piperine and gallic acid are of different chemical natures — piperine is an alkaloid, while gallic acid a phenolic compound. They are used as marker compounds in many plant-based formulations. A highperformance thin-layer chromatographic (HPTLC)-densitometric method has been developed and validated for the simultaneous quantification of piperine and gallic acid as such and in pharmaceutical dosage forms. Toluene-ethyl acetate (3:7) was used as mobile phase and scanning was done at 254 and 340 nm. The method was validated with respect to linearity, reproducibility, specificity, accuracy, precision, robustness, and ruggedness. Both compounds showed good linearities in the range of 250–1750 ng. LOD and LOQ for piperine were 9.98 and 33.29 ng, while for gallic acid 25 and 83.33 ng. Average % RSD values of precision for piperine and gallic acid were 0.46% and 0.72%, respectively. % Recovery was 96–103%. The method is accurate, reproducible, cost-effective, and can be used in routine analysis.

Restricted access

A new method, involving the use of high-performance thin-layer chromatography, has been developed, which detects 10–20% (w/w) of adulterant tallow in cow ghee. The method consists of high-performance thin-layer chromatographic separation of both unsaponifiable and saponified portions using n-hexane-diethyl ether-glacial acetic acid (4:6:0.2, v/v) (for unsaponifiable portion) and n-hexane-diethyl ether-glacial acetic acid (6.5:3.5:0.2, v/v) (for saponifiable portion) as mobile phases. Beef tallow was mixed with cow ghee in various proportions (5 to 90%) to obtain admixtures of beef tallow with cow ghee. The analysis of the samples of cow ghee containing different proportions of beef tallow revealed that the addition of beef tallow to cow ghee affected the chromatographic profile; the effect increased with increasing proportions of beef tallow. Ghee adulterations with tallow at levels down to 20% are clearly seen visually in the chromatographic profile.

Restricted access