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Journal of Radioanalytical and Nuclear Chemistry
Author:
S. Javaid Khurshid
Abstract
Estimation of vitamin B12 in blood is very important to determine in decifiency and diagnosis of anemic patients. Vitamin B12 in blood can be estimated by spectrochemical, enzymatic, radioisotopic and microbiological methods. In the present study vitamin B12 was determined in 48 normal subjects of Rawalpindi/Islamabad by radiometric microbiological assay (RMA) technique using a very rapid, sensitive and automated instrument Bactec 460. In this procedure14C-glucose media and microorganismsLactobacillus leichmannii were used. The sensitivity of the method for vitamin B12 is 1 pg/ml and the vitamin B12 found in normal subjects was in the range of 105–535 pg/ml with a median value of 246±6 pg/ml.
