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  • Author or Editor: Á. Gutermuth x
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Blossom blight caused by Monilia laxa (Ehr.) is the most important fungal disease in Hungarian apricot orchards. The cultivars traditionally grown in the country are susceptible to Monilia laxa (Ehr.) to various extents. In this study the shoots of one tree each of the varieties Zard and Korai Zamatos and 48 hybrids from their cross were artificially infected in vivo with Monilia laxa (Ehr.). The results indicated that when artificial infections are made to evaluate pathogen resistance, this should be carried out on one-year-old shoots, since this is the natural infection point of Monilia . It also appears that, due to the great variability in the size of destroyed tissues, the microscopic analysis of the infections could provide a more reliable evaluation of progeny resistance than comparing the sizes of destroyed shoot areas.

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Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

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