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Abstract  

An improved method for the synthesis of four heterotrinuclearpolyacids of the type: Hx[EM′yM″zO40nH2O (E=P, Si; M′=Mo, W; M″=V, W) was elaborated. The studied compounds were characterized by elemental analysis, IR spectra and thermal behaviour over 20–800C temperature range.

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Acta Alimentaria
Authors: B. Vecseri-Hegyes, P. Fodor, and Á. Hoschke

Fermentation of wort is the most critical step of beer production. Fermentation difficulties may be caused by inadequate wort composition, i.e. insufficient supply of trace elements. Wort provides trace elements for yeast. Among them zinc, which is essential for beer fermentation, is not available in the required amount in wort. Zinc utilization in fermentation was studied in beers made with adjunct (maize, barley). Inductively coupled plasma-atomic emission spectrometry was used as analytical method for the determination of zinc concentration. Addition of zinc made the fermentation faster. The best result was achieved at 0.4 mg l-1. Synthesis of aroma compounds was also promoted at this concentration. Concentrations higher than 0.4 mg l-1 did not affect significantly either the fermentation rate or the maximal ethanol concentration. Addition of zinc at the end of wort boiling did not prove to be practical, it is better to supply yeast with zinc.

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Acta Alimentaria
Authors: B. Vecseri-Hegyes, P. Fodor, and Á. Hoschke

The most sensitive and time-consuming technological step of beer production is the fermentation of wort. Problems during fermentation will not only prolong production time but it will lead to the deterioration of beer quality. Most often it is due to low zinc concentration of the wort or the yeast. Due to lack of zinc the fermentation lasts longer, composition of fermentation by-products changes, maltose intake slows down, sedimentation ability and heat sensitivity of the yeast increase. In Part I of our work, the factors affecting the degree of zinc supply of wort were examined. During the examination of zinc supply of wort two kinds of beer were produced: all-malt beer and beer with adjunct. Change of zinc concentration was followed throughout the brewing process at every technological step. It was found that concentration of zinc gradually decreases during production of wort, and only a small fraction of the calculated amount appears in it. Wort made with adjunct has even lower zinc concentration than all-malt wort. In all malt wort 3.4%, while in wort containing adjunt only 0.4% of the zinc appeared in the wort. Yeast can absorb only the ionic form of zinc during fermentation, thus we had elaborated a method for the separation of the organic and inorganic form of zinc, which was followed by the determination of the concentration of ionic zinc in wort prior to fermentation.

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Previously, we sequenced the HSV-1 Ul39-Ul40 homologue genes of Aujeszky's disease virus (ADV), also designated as pseudorabies virus (Kaliman et al., 1994ab). Now we report the nucleotide sequence of the adjacent DNA that encodes Ul38, the 5'-region (750 bp) of Ul37, and the promoter regions between these divergently arranged two genes. The ADV Ul38 gene encodes a protein of 368 amino acids. Amino acid sequence comparison of ADV Ul38 with that of other herpesviruses revealed significantstructural homology. In a transcription study using RNase protection assay and Northern blot hybridizationwe found that the Ul38 gene had one initiation site, but the Ul37 gene was initiated at two transcription sites with two potential initiator AUGs, one of which was dominant. Comparison of ADV Ul37, Ul38 and ribonucleotide reductase gene expression showed that these genes belong to the same temporal class with early kinetics. Data of structural and transcriptional studies suggest that regulation of the expression of these two ADV genes could differ from that of the HSV-1 virus.

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Abstract  

An improved method for the synthesis of two heteropolyacids of the same type: H5[AsMo10V2O40]·13H2O and H5[AsW10V2O40]·16H2O was elaborated. The studied compounds were characterized by elemental analysis, IR spectra and thermal behaviour over 20-800°C temperature range.

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One of the plasmids present in a Haemophilus somnus strain isolated from nasal discharge of a cattle with respiratory disease was purified and cloned for DNA sequencing. The plasmid was found to be 1065 base pairs long with 39.2% G+C content, and showed no homology to any DNA sequenced so far. It has no capacity to code any protein longer than 43 residues. It is not clear yet if this plasmid codes Haemophilus somnus specific factors.

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In the present study, 16 women with recurrent vulvovaginal candidiasis (RVVC) due to Candida albicans and Candida (Torulopsis) glabrata were followed for a period of 4 to 12 months, and 36 vaginal isolates were evaluted by pulsed-field gel electrophoresis (PFGE). Eleven women were infected by C. albicans and5 by C. glabrata.Three electrophoretic karyotypes of C. albicans and 3 of C. glabrata were identified throughout the follow-up. All patients but one was infected with the same karyotype of C. albicans or C. glabrata during the follow-up period.Two different karyotypes of C. glabrata were identified in one patient in the course of 12 months. The results confirmed the diversity of the karyotypes of C. albicans and C. glabrata causing vulvovaginitis and demonstrated the persistence of colonization with the same strain over different periods of time despite therapy (15/16 women).

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Acta Physiologica Hungarica
Authors: Daniela Fodor, A. Albu, L. Poantă, and M. Porojan

The role of vitamin K in the synthesis of some coagulation factors is well known. The implication of vitamin K in vascular health was demonstrated in many surveys and studies conducted over the past years on the vitamin K-dependent proteins non-involved in coagulation processes. The vitamin K-dependent matrix Gla protein is a potent inhibitor of the arterial calcification, and may become a non-invasive biochemical marker for vascular calcification. Vitamin K 2 is considered to be more important for vascular system, if compared to vitamin K 1 . This paper is reviewing the data from recent literature on the involvement of vitamin K and vitamin K-dependent proteins in cardiovascular health.

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The association between metabolic syndrome (MS) and bone status remains controversial. We aimed to study the relationships between MS, bone mineral density (BMD), and bone metabolism in postmenopausal women.

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