You are looking at 1 - 10 of 17 items for
- Author or Editor: A. Blázovics x
- Refine by Access: All Content x
Culinary herbs and medicinal plants could be used as natural food ingredients to replace synthetic antioxidants. Two natural antioxidant preparations, namely Rosmol (liquid) and Rosmol-P (powder) were produced by extraction from a mixture of medicinal plants belonging to the Lamiaceaefamily, such as rosemary (Rosmarinus officinalis L.),self heal(Prunella vulgaris L.)hyssop(Hyssoppus officinalis L.)and lemon balm (Melissa officinalis L.). The main active compound of the extract is supposed to be a phenolic (caffeic) acid derivative. The total polyphenol content of the preparations is very high, 8.72 g l-1for Rosmol and 93.7 g kg-1for Rosmol-P. The products acted as primary and secondary antioxidants, chelating transitional metal ions and inhibiting the autoxidation of linoleic acid. Rosmol and Rosmol-P scavenged free radicals formed during Fenton type reaction measured by chemiluminometry, and also exhibited strong antioxidant property in Randox TAS measurement. The antioxidant activity of the products was unchanged after six months of storage. According to the in vitro studies, the products are suitable to use as antioxidant ingredients instead of synthetic ones in lipid containing food products.
Magnesium participates in numerous enzymatic reactions in the human body and it has essential role in the maintenance of the antioxidant system. Since several magnesium compounds have been applied in the food and pharmaceutical industry, our purpose was to investigate the antioxidant/free radical scavenging activity of some magnesium compounds in vitro. The antioxidant/prooxidant effect of inorganic salts (e.g. MgCl2) and organic complexes (e.g. Mg-gluconate) was determined with chemiluminometric method (H2O2/•OH-microperoxidase-luminol) and heme mediated LDL oxidation (LDL-heme-H2O2 ) in vitro. It has been stated that the chemiluminescence method and LDL (low density lipoprotein) oxidation measurement is applicable in the presence of magnesium salts and complexes. Most of the compounds do not generate free radicals and the antioxidant/prooxidant effect depends on the quality of the ligand and the concentration. In the concentration range used, some representatives of the magnesium compounds (MgO, Mg-gluconate, Mg-polygalacturonate) investigated showed radical generating activity measured with chemiluminescence method, whereas the LDL oxidation has not been affected. Magnesium citrate and malate proved to be antioxidants measured with the chemiluminescence method and they slightly accelerate the LDL oxidation in the system and in the concentration applied. In vitro some of the ligands of magnesium compounds showed antioxidant activities.
Free radical production and disturbance in the redox status may modulate the expression of a variety of immune and inflammatory molecules. Antioxidants could inhibit the proliferation of T lymphocytes induced by mitogens. The aim of this study was to investigate the effect of the infusion of dried calyx of Hibiscus sabdariffa L. on T-cell-mediated immune response. We examined the effect of the infusion on spontaneous and mitogen-induced blast transformation of human lymphocytes in vitro. Peripheral blood lymphocytes from heparinised venous blood of healthy subjects (n=17) were examined. Five and 10 mg ml-1 phytohemagglutinin (PHA) and 5 mg ml-1 pokeweed mitogen (PWM) were added. Depending on the concentration, calyx infusion of H. sabdariffa decreased mitogen-induced blastogenesis in normal subjects. It did not affect, however, the level of spontaneous proliferation of human lymphocytes in vitro. The infusion of hibisci flos may alleviate over-stimulated condition of the immune system in hypersensitivity conditions.
In in vitro studies it was detected that the juice from the choleretic black radish root had hydrogen donating and d-field element-chelating abilities. The juice exhibited strong reducing power property and radical scavenging effect in H2O2/∙OH luminol system.
With the help of short term animal experiment we examined the in vivo effect of Raphanus sativus root juice on hyperlipidemia in rats.
A chemiluminescent study was applied to determine the total scavenger activity (TSC) of erythrocytes and plasma of untreated and treated animals. We also determined the different liver enzyme activities: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and metabolite concentrations (cholesterol, triacylglycerols, total bilirubin, glucose) by spectrophotometry in the sera.
In our study, using experimental hyperlipidemic condition, it has been detected that black radish root juice could moderate the injurious effect of lipid rich diet in rats in vivo.
The different methods of making herbal tea used in various cultures may decrease the efficiency of herbal tea, therefore the purpose of the present study was to examine the changes in antioxidant activity and metal ion concentration in aqueous extracts of “Tieguanyin Stomach Tea” obtained with different steeping times. Partial phytochemical examination, element analysis, and the determination of antioxidant properties were carried out. High Al, Cr, Mn, Ni, Pb and Ti content was observed in the tea drug. Aqueous extracts of the drug sample showed H-donor activity, reducing power and scavenging activity, depending on the steeping time and concentration. According to our findings, 5-min steeping was the least effective and redox parameters of the aqueous extracts did not change considerably between 15 and 120 min of steeping time.
Chronic gastrointestinal diseases are mostly attached to inflammation as well as modified redox homeostasis. These diseases cause remarkable health problems in the northern regions of Europe, but as the climate becomes colder, the harvesting of fruit with notable antioxidant content becomes harder. In these regions, polyphenol-rich berries, which can handle the climate, may have pivotal role in the diet. The aim of our study was to determine antioxidant properties of watery extracts of blueberry, Vaccinium corymbosum L., bilberry, V. myrtillus L., and lingonberry, V. vitis-idaea L. that may have role in the redox homeostasis of the gastrointestinal region. Data show that these berries notably inhibit the lipid peroxidation, underpinned by reducing power, total polyphenol and ascorbic acid content. The study demonstrates that berries can play a remarkable role in northern European diet, especially when redox homeostasis is imbalanced.
An experimental surgical model was developed in rats after a short term alimentary induced hyperlipidemy to study the direct effect of bile on the colonic mucosa, with regard to the cancerogenic properties of lipid rich diet. The purpose of this study was to light on the role of fatty acid alteration and lipid peroxidation processes of bile in the epithelial cell damage. Animals were fed with normal (group A) and fat rich diet (group B) for 10 days and then bile samples were collected by the cannulation of the common bile duct in deep anaesthesia. The circulation preserved colons of control rats were treated either with bile from the control or hyperlipidemic rats. The treatment was carried out for 30 minutes. The electronmicroscopic alterations of epithelial cells (both enterocytes and goblet cells) caused by bile from hyperlipidemic rats were significantly greater than that of controls. Unfavourable changes of the redox state of the colonic mucosa were also detected both in the hyperlipidemic and bile treated groups. A significant increase was observed in the free-SH concentration of the two bile treated groups against the untreated animals. The changes could be explained among others by the modified bile fatty acid composition. The present study supports that the alimentary modified bile can influence the structure of the epithelium of colonic mucosa and it can be one of the inducing factor of carcinogenesis.