Authors:A. Lugasi, J. Hóvári, K. Hagymási, I. Jakóczi, and A. Blázovics
Culinary herbs and medicinal plants could be used as natural food ingredients to replace synthetic antioxidants. Two natural antioxidant preparations, namely Rosmol (liquid) and Rosmol-P (powder) were produced by extraction from a mixture of medicinal plants belonging to the Lamiaceaefamily, such as rosemary (Rosmarinus officinalis L.),self heal(Prunella vulgaris L.)hyssop(Hyssoppus officinalis L.)and lemon balm (Melissa officinalis L.). The main active compound of the extract is supposed to be a phenolic (caffeic) acid derivative. The total polyphenol content of the preparations is very high, 8.72 g l-1for Rosmol and 93.7 g kg-1for Rosmol-P. The products acted as primary and secondary antioxidants, chelating transitional metal ions and inhibiting the autoxidation of linoleic acid. Rosmol and Rosmol-P scavenged free radicals formed during Fenton type reaction measured by chemiluminometry, and also exhibited strong antioxidant property in Randox TAS measurement. The antioxidant activity of the products was unchanged after six months of storage. According to the in vitro studies, the products are suitable to use as antioxidant ingredients instead of synthetic ones in lipid containing food products.
Authors:K. Szentmihályi, M. Szilágyi, J. Balla, L. Ujhelyi, and A. Blázovics
Magnesium participates in numerous enzymatic reactions in the human body and it has essential role in the maintenance of the antioxidant system. Since several magnesium compounds have been applied in the food and pharmaceutical industry, our purpose was to investigate the antioxidant/free radical scavenging activity of some magnesium compounds in vitro. The antioxidant/prooxidant effect of inorganic salts (e.g. MgCl2) and organic complexes (e.g. Mg-gluconate) was determined with chemiluminometric method (H2O2/•OH-microperoxidase-luminol) and heme mediated LDL oxidation (LDL-heme-H2O2 ) in vitro. It has been stated that the chemiluminescence method and LDL (low density lipoprotein) oxidation measurement is applicable in the presence of magnesium salts and complexes. Most of the compounds do not generate free radicals and the antioxidant/prooxidant effect depends on the quality of the ligand and the concentration. In the concentration range used, some representatives of the magnesium compounds (MgO, Mg-gluconate, Mg-polygalacturonate) investigated showed radical generating activity measured with chemiluminescence method, whereas the LDL oxidation has not been affected. Magnesium citrate and malate proved to be antioxidants measured with the chemiluminescence method and they slightly accelerate the LDL oxidation in the system and in the concentration applied. In vitro some of the ligands of magnesium compounds showed antioxidant activities.
Authors:D. Kleiner, D. Kurucz, A. Bersényi, K. Szentmihályi, A. Skesters, L. Zuka, and A. Blázovics
Chronic gastrointestinal diseases are mostly attached to inflammation as well as modified redox homeostasis. These diseases cause remarkable health problems in the northern regions of Europe, but as the climate becomes colder, the harvesting of fruit with notable antioxidant content becomes harder. In these regions, polyphenol-rich berries, which can handle the climate, may have pivotal role in the diet. The aim of our study was to determine antioxidant properties of watery extracts of blueberry, Vaccinium corymbosum L., bilberry, V. myrtillus L., and lingonberry, V. vitis-idaea L. that may have role in the redox homeostasis of the gastrointestinal region. Data show that these berries notably inhibit the lipid peroxidation, underpinned by reducing power, total polyphenol and ascorbic acid content. The study demonstrates that berries can play a remarkable role in northern European diet, especially when redox homeostasis is imbalanced.
Authors:E. Rapavi, K. Szentmihályi, A. Lugasi, E. Vági, É. Bányai, A. Balázs, É. Szőke, and A. Blázovics
The different methods of making herbal tea used in various cultures may decrease the efficiency of herbal tea, therefore the purpose of the present study was to examine the changes in antioxidant activity and metal ion concentration in aqueous extracts of “Tieguanyin Stomach Tea” obtained with different steeping times. Partial phytochemical examination, element analysis, and the determination of antioxidant properties were carried out. High Al, Cr, Mn, Ni, Pb and Ti content was observed in the tea drug. Aqueous extracts of the drug sample showed H-donor activity, reducing power and scavenging activity, depending on the steeping time and concentration. According to our findings, 5-min steeping was the least effective and redox parameters of the aqueous extracts did not change considerably between 15 and 120 min of steeping time.
Authors:I. Kocsis, A. Lugasi, K. Hagymási, Á. Kéry, J. Fehér, É. Szõke, and A. Blázovics
In in vitro studies it was detected that the juice from the choleretic black radish root had hydrogen donating and d-field element-chelating abilities. The juice exhibited strong reducing power property and radical scavenging effect in H2O2/∙OH luminol system.
With the help of short term animal experiment we examined the in vivo effect of Raphanus sativus root juice on hyperlipidemia in rats.
A chemiluminescent study was applied to determine the total scavenger activity (TSC) of erythrocytes and plasma of untreated and treated animals. We also determined the different liver enzyme activities: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and metabolite concentrations (cholesterol, triacylglycerols, total bilirubin, glucose) by spectrophotometry in the sera.
In our study, using experimental hyperlipidemic condition, it has been detected that black radish root juice could moderate the injurious effect of lipid rich diet in rats in vivo.
Authors:P. Sipos, K. Hagymási, A. Lugasi, Edit Fehér, F. Örsi, and A. Blázovics
An experimental surgical model was developed in rats after a short term alimentary induced hyperlipidemy to study the direct effect of bile on the colonic mucosa, with regard to the cancerogenic properties of lipid rich diet. The purpose of this study was to light on the role of fatty acid alteration and lipid peroxidation processes of bile in the epithelial cell damage. Animals were fed with normal (group A) and fat rich diet (group B) for 10 days and then bile samples were collected by the cannulation of the common bile duct in deep anaesthesia. The circulation preserved colons of control rats were treated either with bile from the control or hyperlipidemic rats. The treatment was carried out for 30 minutes. The electronmicroscopic alterations of epithelial cells (both enterocytes and goblet cells) caused by bile from hyperlipidemic rats were significantly greater than that of controls. Unfavourable changes of the redox state of the colonic mucosa were also detected both in the hyperlipidemic and bile treated groups. A significant increase was observed in the free-SH concentration of the two bile treated groups against the untreated animals. The changes could be explained among others by the modified bile fatty acid composition. The present study supports that the alimentary modified bile can influence the structure of the epithelium of colonic mucosa and it can be one of the inducing factor of carcinogenesis.
Authors:L. Váli, H. Fébel, É. Stefanovits-Bányai, É. Sárdi, A. Lugasi, K. Szentmihályi, and A. Blázovics
Dietary intake of
polyphenols and vitamins before surgery may have beneficial effect on survival
of the patients. Table beet (Beta
vulgaris L. var. rubra) contains
bioactive agents, which have a wide range of different physiologic effects.
These agents have beneficial antioxidant properties. The aim in the present
study was to determine the gut protecting properties of bioactive substances of
table beet in a model of ischaemic-reperfusion injury of the rat liver.
Chemiluminescent intensity, H-donating ability, reducing power and free
SH-group concentracion were determined by luminometry and spectrophotometry in
duodenum and plasma. Lyophilized table beet increased significantly the free
radical scavenging property of the plasma measured by chemiluminometry. Other
antioxidant parameters of the plasma increased in rats fed table beet diet, and
change in reducing power was significant. Beneficial effect of the treatment
was found in changes of total scavenger capacity of the duodenum during
ischaemia-reperfusion injury. H-donating ability and reducing power of the gut
decreased in the table beet-fed group during ischaemia-reperfusion compared to
normal group with ischaemia-reperfusion. Based on these data, it can be
concluded that table beet can protect the entire body from the oxidative damage
caused by ischaemia-reperfusion of the liver, but the effect of table beet
treatment on gut mucosa needs further investigation.