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Acta Microbiologica et Immunologica Hungarica
Authors: Ildikó Faludi, Ágnes Szabó, Katalin Burián, Valéria Endrész, and A. Miczák

Mycobacterium smegmatis is a species of rapidly growing saprophytes with a number of properties that make it an effective vaccine vector. Recombinant M. smegmatis expressing protective antigens of different pathogens and molecules modulating the immune responses offers some potential for reduction of the burden of tuberculosis, HIV and hepatitis B infections. This paper discusses the molecular methods used to generate recombinant M. smegmatis and the results obtained with some of these recombinants.

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Abstract  

The properties of miniature therapeutic silicon photodiodes in a 14.7 MeV field are outlined in the paper. The responses of the photodiode to the tissue dose rate of both the photons and 14.7 MeV neutrons are given. The decrease in sensitivity due to radiation damage is specified for a n- field.

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Purpose

Suprapubic bladder aspiration is an invasive procedure in which a needle is used to obtain a urine sample directly from the bladder. Its advantages are sensitivity (detection of significant bacteriuria is close to 100%), suitability for cultivation of anaerobic bacteria, and low risk of contamination. Our purpose was to characterize the microbiology and epidemiology of urine samples obtained through this procedure in the Clinical Center of the University of Szeged’s Institute of Clinical Microbiology between 2008 and 2017.

Materials and methods

Over the 10-year period, patient data were collected and suprapubic bladder aspirations were performed, and the samples are processed in accordance with routine laboratory procedures in clinical bacteriology.

Results

Of 187 urine samples obtained from 148 patients, 32.6% (n = 61) were culture-positive (defined as 102 colony forming units/ml or more).

Conclusions

This method should be considered an important sampling procedure in the differential diagnostics of upper urinary tract infections, particularly in children <2 years of age, and in older people, hospitalized patients.

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Acta Microbiologica et Immunologica Hungarica
Authors: Ildikó Faludi, Ágnes Csanádi, Ágnes Szabó, Katalin Burián, Valéria Endrész, and A. Miczák

Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein.

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Journal of Radioanalytical and Nuclear Chemistry
Authors: K. Prokeš, A. Burian, L. Judas, P. Kasík, R. Keclík, B. Konopásek, and L. Petruželka

Abstract  

Miniature silicon photodiodes have been tested for utilization in radiotherapy. Their use in the treatment of ca laryngis is described. The actually given dose was monitored during irradiation. Treatment plan or the setting up of the entrance fields was modified according to the response of the diode. This method seems to be a useful tool for checking both the treatment plan and appropriate setting up of the patient in treatment position.

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