In the collections accumulated within the framework of the Flora of Guerrero Project appeared a remarkable Psychotria taxon belonging to the large and rather polymorphic species of P. horizontalis Sw. but differs from both known subspecies of it, which are the type and the subsp. glaucescens (Kunth) Borhidi, with discolourous leaves and completely glabrous inflorescences. The authors consider this new taxa as a third subspecies under the name P. horizontalis subsp. glaucina Borhidi et R. M. Fonseca.
Authors:A. Fonseca, J. Frydman, Vanessa Rocha, and M. Bernardo-Filho
Acetylsalicylic acid is the most widely used drug as antipyretic, analgesic, anti-inflammatory agent and for secondary prevention of thrombotic phenomena in the heart, brain and peripheral circulation. Drugs can modify the labeling of blood constituents with technetium-99m (
Tc). This work has evaluated the effect of
treatment with acetylsalicylic acid on the
labeling of the blood constituents with
Tc. Wistar rats were treated with different doses (1.5, 3.0 and 6.0 mg/kg) of acetylsalicylic acid during 1 hour. At higher dose used (6.0 mg/kg) animals were treated during different period of time (0.25, 1.0 and 4.0 hours). Animals treated with physiologic saline solution were used as control. After the labeled process; plasma (P), blood cells (BC), insoluble (IF-P, IF-BC) and soluble (SF-P, SF-BC) fractions were separated. Afterwards, the percentage of radioactivity (%ATI) in each fraction was calculated. The treatment during 1 hour with acetylsalicylic acid at higher dose has significantly (p<0.05) modified the fixation of
Tc on blood cells. Considering the results, we suggest that acetylsalicylic acid used at therapeutic doses may interfere with the nuclear medicine procedures related to these blood constituents.
Authors:Gabrielle Souza Rocha, A. Fonseca, Michelle Rodrigues, F. Dantas, A. Caldeira-de-Araujo, and R. Santos
The aim of this work was to evaluate, by comet assay, the possible inducing of DNA lesions in peripheral blood mononuclear cells of rats subjected to acute or chronic food deprivation. Wistar male rats were subjected to 72 h of partial (50%), or total acute food deprivation, and then allowed to recover for different time periods (24, 48 and 72 h). In other experiments, comet scores were determined in peripheral blood mononuclear cells of rats subjected to chronic food deprivation (25% and 50%) for 50 days. Blood aliquots were obtained before, during and after food deprivation. Comet assay was carried out, the comet units photographed and scored (class 0 up to 3). Acute and chronic food-deprived rats presented peripheral blood mononuclear cells with DNA lesions (comet classes 1, 2 and 3) and a significant increase (
< 0.05) in the number of comet units compared with its basal level. The increase was proportional to acute food deprivation time, but after being taken off, it progressively returned to basal level after 48 h (partial group) or 72 h (total group). Chronic food-deprived rats presented a progressive increase of comet score up to 5 days, and a decrease thereafter to reach a basal level. Possible mechanisms of DNA lesions are discussed.
Authors:Luiza Arakaki, Josiane Diniz, A. Silva, Vera Augusto Filha, Maria Fonseca, J. Espínola, and T. Arakaki
The divalent copper, nickel, cobalt and trivalent chromium, molybdenium and iron chelate compounds derived from bis(acetylacetone)
ethylenediimine were grafted on activated silica gel using a batch process in methanolic solution. The sequence of the maximum
retention capacity was Cr(III)>Mo(III)>Fe(III)>Co(II)>Ni(II)>Cu(II). Calorimetric titration was employed to study the interaction
of activated silica gel with these series of metal chelate compounds. Exothermic enthalpic results were obtained throughout
all interactions process. The spontaneity of these systems was reflected in negative and positive free Gibbs energy from entropic
Authors:Luiza Arakaki, Maria da Fonseca, J. Espínola, A. Wanderley, E. Martins, T. Arakaki, and C. Airoldi
The reaction of cyclic ethylene sulfide with 3-aminopropyltrimethoxysilane resulted in a new silylating agent, which was anchored
onto a silica surface via the sol-gel procedure using hydrochloric acid or ammonium hydroxide as acid or base catalysts, respectively.
Both organofunctionalized surfaces have the ability to adsorb divalent cations from aqueous nitrate solution. Through calorimetric
titrations, the thermodynamic data of cation-basic center interactions were obtained. Exothermic enthalpy values were obtained
for all interaction processes. The calculated free Gibbs energy is in agreement with the spontaneity of the reactions and
the positive entropy values for all reactions are favorable for these systems.
Authors:B. Matos, E. Aricó, M. Linardi, A. Ferlauto, E. Santiago, and F. Fonseca
Thermal analysis has been used to evaluate the stability, glass transition, and water retention of Nafion based polymer–ceramic
electrolytes. These electrolytes are envisioned as promising replacement of Nafion in fuel cells operating above 100 °C. The
polymeric matrix prepared by casting exhibits lower crystallinity than the extruded Nafion, a feature that affects the water
absorption properties. The addition of titania-based nanotubes and nanoparticles to the polymer has enhanced the water retention
at high temperatures (~130 °C) and the glass transition temperature, respectively. Such results are important for the design
of composite electrolytes for the operation of fuel cells at high temperatures.
Authors:E. Arico, F. Tabuti, F. Fonseca, D. de Florio, and A. Ferlauto
The thermal behavior of the yttria-stabilized zirconia (YSZ) and nickel oxide (YSZ–NiO) composite mixtures with the addition
of graphite, multiwall carbon nanotubes and functionalized multiwall carbon nanotubes was studied. The YSZ–NiO composite is
the precursor of the YSZ–Ni anode of solid oxide fuel cells. The anode exhibits a porous structure, which is usually obtained
by the addition of carbon containing pore formers. Thermal analysis and X-ray diffraction evidenced that the properties of
carbonaceous materials (C) and atmosphere have a strong influence on the thermal evolution of the reactions taking place upon
heating the anode precursor. The dependence of both the carbon content and the chemical nature of the ceramic matrix on the
thermal behavior of the composite were investigated. The discussed results evidenced important features for optimized processing
of the anode.
Authors:J. Díaz De León, R. Escoppinichi, R. Zavala-Fonseca, T. Castellanos, M. Röder, and A. Mujeeb-Kazi
To determine limits of tolerance, provide information about genetic diversity, and explore potential as progenitors for a salt-tolerant wheat improvement program, we collected several landraces and genotypes reputed to be salt-tolerant. Salt tolerance was tested by irrigation with a diluted solution of seawater with 12 dS.m
electrical conductivity for two years. Phenotypic parameters of percent of emergence, days to flowering to spike emergence, and physiological maturity were not significantly affected. Leaf area was sensitive to salt stress and inhibited about 30%. Plant height was inhibited 30%, while spike length and number of grains per spike were not. Total yield of Shorawaki and Kharchia landraces confirmed their reputation as salt-tolerant. Cultivars Mepuchi, Pericu, Calafia, WH157, and SNH-1 were inhibited at a moderate level of tolerance; cultivars Cochimí, Lu26S, and KRL 1–4 were inhibited, as was the control cultivar Oasis by up to 50%. To amplify microsatellites from genomes A, B, and D, 33 pairs of primers were used. The microsatellite WMS169-6A was highly polymorphic, with 10 different alleles distinguishing the genotype set. Also, the short arm of chromosome 4D microsatellites were amplified and found to be monomorphic, which suggests highly conserved alleles. The other microsatellites had variable polymorphism. In total, 120 alleles were obtained and used to define genetic diversity. The resulting dendrogram showed that landraces Shorawaki and Kharchia are distantly grouped from all other cultivars, as well as the cultivar Chinese Spring. Strikingly, KRL1–4, a derivative of Kharchia, did not show a close relationship to its source. The geographic origin did not influence pair-wise combinations. However, pedigree did influence pair-wise combinations.
Authors:A. S. Fonseca, J. N. G. Frydman, R. Santos, and M. Bernardo-Filho
Acetaminophen (AAP), acetylsalicylic acid (ASA) and dipyrone (DIP) are antipyretic and analgesics drugs that have wide use in health sciences. Some drugs can modify the labeling of blood elements with technetium-99m (99mTc). This work has evaluated the effect of AAP, ASA and DIP on the labeling of the blood elements with 99mTc. Blood was incubated with different concentrations of the drugs before the 99mTc-labeled process. Plasma (P), blood cells (BC), insoluble (IF-P, IF-BC) and soluble (SF-P, SF-BC) fractions were separated and percentage of radioactivity (%ATI) in each fraction was determined. Data have shown that the antipyretic drugs used in this study did not significantly modify the fixation of 99mTc on the blood elements when the experiments were carried out with the doses usually used in human beings. Although the experiments were carried out with rats, it is possible to suggest that AAP, ASA or DIP should not interfere with the procedures in nuclear medicine involving the labeling of blood elements with 99mTc