In 2005–2008 virulence of the wheat leaf rust population was studied on Thatcher near-isogenic lines with
Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26
. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 233 wheat leaf rust isolates were analyzed. Resistance gene
was effective to all tested isolates as in the four previous years. Virulence to
(0.8% of the analysed isolates) was found. Gene
conditioned resistance to almost all collections and a lower frequency of virulence was also observed on
. Twenty-six winter wheat cultivars, seven spring wheat cultivars and seven winter triticale cultivars registered in 2005–2008 were tested with 7 leaf rust isolates. Winter wheat cultivars Biscay, Mulan and Orlando were resistant to all isolates and were also resistant in field trials.
In 2013–2015 virulence in the wheat leaf rust population was evaluated on 17 Thatcher near-isogenic lines with leaf rust resistance genes. A total of 110 wheat leaf rust isolates were analyzed. Resistance genes Lr9 and Lr19 were effective to all tested isolates. Genes Lr24 and Lr28 conditioned resistance to 92% of the tested rust isolates. Thirty-seven winter wheat cultivars registered in Slovakia were analyzed for the presence of Lr10, Lr24, Lr26, Lr34 and Lr37 using tightly linked molecular markers. Gene Lr37 was the most common in the tested cultivars. Leaf rust resistance was also tested in 13 wheat cultivars at the seedling stage with representative pathotypes of leaf rust.