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In the present study, 16 women with recurrent vulvovaginal candidiasis (RVVC) due to Candida albicans and Candida (Torulopsis) glabrata were followed for a period of 4 to 12 months, and 36 vaginal isolates were evaluted by pulsed-field gel electrophoresis (PFGE). Eleven women were infected by C. albicans and5 by C. glabrata.Three electrophoretic karyotypes of C. albicans and 3 of C. glabrata were identified throughout the follow-up. All patients but one was infected with the same karyotype of C. albicans or C. glabrata during the follow-up period.Two different karyotypes of C. glabrata were identified in one patient in the course of 12 months. The results confirmed the diversity of the karyotypes of C. albicans and C. glabrata causing vulvovaginitis and demonstrated the persistence of colonization with the same strain over different periods of time despite therapy (15/16 women).

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Vitamin D2 is essential for the human body and mushrooms are proved to be one of the best sources of it. In our experiment biologically active, pre-harvest white and cream type button mushrooms and oyster mushroom were treated with UVB light in order to increase their vitamin D2 level, by transforming their natural ergosterol content into vitamin D2. UVB lamps (operating on 312 nm) and six time periods of irradiation (15 to 90 min) were used. After three consecutive days of treatments the yield were measured and samples were taken for vitamin D2 analysis. Data showed considerable increase in vitamin D2 levels at every time period in each cultivar.

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Abstract  

A new terminology of Mössbauer pattern analysis is introduced. In this approach Mössbauer parameters are considered as functions of a number of externally adjusted physical parameters at which the spectrum has been recorded. Our approach can also help to systematize Mössbauer data for the identification of individual physical or chemical species from the corresponding patterns present in the spectrum.

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Abstract  

For the electrodeposition of119Sn, aluminium, iron, nickel, copper, silver and platinum foil substrates were used. The samples were measured before and after heat-treatments between 350–485 K. In several cases interphase formation was observed even before the first heat treatment. Diffusion data were alsc obtained.

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Periodica Mathematica Hungarica
Authors: S. Gyökér, M. Szilvási-Nagy, and Á. Császár

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Studies on transition-metal picoline complexes

I. Preparation and thermoanalytical investigations

Journal of Thermal Analysis and Calorimetry
Authors: G. Liptay, A. Borbély-Kuszmann, and G. Nagy
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Analysis of flagellin genes was carried out on strains of Salmonella Typhimurium, Salmonella Hadar, Salmonella Abortusequi, Salmonella Enteritidis and Salmonella Gallinarum serovars, using a PCR system designed in this study. The purpose of these studies was to explore the flagellin genes of biphasic and monophasic Salmonellae for future targeted genetic interventions. The PCR primers were designed for two different structural genes of flagellin (fliC, fljB), for the repressor of fliC (fljA), for the operator region of fliC, and for the invertase system responsible for phase variation in Salmonella (hin, hixL, hixR). PCR analysis revealed that all of the examined genes (fliC, fliC-operator, fljB, fljA, hin, hixL, hixR) were present in all S. Typhimurium (n = 10)and S. Hadar (n = 10) strains tested. The results obtained on S. Typhimurium and S. Hadar strains confirmed their biphasic character at DNA level. However, the S. Enteritidis (n = 46) and S. Gallinarum (n = 5) strains lacked the invertase system (hin, hixL, hixR) as well as the fljA and fljB genes, while fliC and its operator were detectable. Consequently, the S. Enteritidis strains could only express fliC gene resulting in phase H1 flagellin. The examined S. Gallinarum strains were also demonstrated to have a cryptic flagellin gene (fliC). On the other hand, PCR results on S. Abortusequi (n = 2) indicated that both flagellin genes (fliC, fljB) and the whole phase variation system were present in both strains tested but only the H2 phase gene (fljB) was expressed. The phenotype of these strains could be clarified by motility test and/or by classical flagellar serology. The findings are also substantiated by the results of serovar-specific PCR for S. Typhimurium and S. Enteritidis. In conclusion, the PCR system developed in this study proved to be suitable for characterisation of Salmonella flagellin genes and confirmed serological results regarding all S. Typhimurium, S. Hadar and S. Enteritidis strains. This system could also identify cryptic flagellar genes of S. Abortusequi and S. Gallinarum.

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Orvosi Hetilap
Authors: Tamás Nagykálnai, A. Csaba Nagy, and László Landherr

A mediastinum és a mellkasfal (különösen a bal emlő) sugárkezelése utáni kardiális komplikációk különleges problémát jelentenek, mivel a sugárkezelés során a szívet ért dózisok növekedésével – általában évek múlva – egyre emelkedik az ischaemiás szívbetegségek aránya. A sugárzások okozta cardiotoxicitas gyakorisága az onkológiai kezelés fajtájától és magától a betegtől függ (a sugárkezelés részletei, a beteg életkora, neme, komorbid betegségek, dohányzási szokások stb.). Számos régebbi klinikai vizsgálatban beszámoltak arról, hogy a mellkasfal vagy az emlő sugárkezelése emeli a kardiális megbetegedések számát. A mai modern besugárzási módszerekkel a mellkasfal vagy a bal emlő kezelése már jóval biztonságosabb, mint évtizedekkel ezelőtt volt. A cardiotoxicitas elkerülése érdekében javasolt az emlőrák kemo- és célzott terápiáinak használata esetén a klinikai előírások betartása, a legfejlettebb sugárkezelési technikák alkalmazása, a betegek rendszeres monitorozása, valamint a kardiológusok és onkológusok közötti szoros együttműködés. Orv. Hetil., 2014, 155(23), 897–902.

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