Search Results

You are looking at 1 - 5 of 5 items for

  • Author or Editor: A. Pedryc x
  • Refine by Access: All Content x
Clear All Modify Search

Blossom blight caused by Monilia laxa (Ehr.) is the most important fungal disease in Hungarian apricot orchards. The cultivars traditionally grown in the country are susceptible to Monilia laxa (Ehr.) to various extents. In this study the shoots of one tree each of the varieties Zard and Korai Zamatos and 48 hybrids from their cross were artificially infected in vivo with Monilia laxa (Ehr.). The results indicated that when artificial infections are made to evaluate pathogen resistance, this should be carried out on one-year-old shoots, since this is the natural infection point of Monilia . It also appears that, due to the great variability in the size of destroyed tissues, the microscopic analysis of the infections could provide a more reliable evaluation of progeny resistance than comparing the sizes of destroyed shoot areas.

Restricted access

Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

Restricted access

The biotic and abiotic stresses are the major limiting factors in plant productivity. To overcome these difficulties molecular breeding methods have recently been widely used to improve the stress and disease resistance of grapevine cultivars. Crown gall disease caused by Agrobacterium vitis or Agrobacterium tumefaciens causes serious damage worldwide on grapevine, and there is no efficient method yet that can be routinely used by grape-growers to prevent this disease. Therefore genetic manipulation for crown gall resistance would have a great economic impact. To this end embryogenic culture of Vitis berlandieri × Vitis rupestris cv. Richter 110 was transformed with a virE1 gene construct. Twenty-six plant lines were selected, and their transgenic nature was confirmed by PCR analysis. Seventeen of the 26 lines showed resistance to crown gall disease following inoculation with A. vitis Tm4 strain.

Restricted access

To raise the efficiency of plant regeneration we studied the important and necessary elements of the procedure. The embryogen capacity of 33 various grape genotypes were tested on four different induction media. We successfully obtained anther derived embryogenic calli in 27 genotypes with the range of 1–12%, this is the first reported protocol for embryogenesis from Korai bíbor, Odysseus, Orpheus and Pannon frankos cultivars. Two sorts of sterilization treatments were examined before inducing somatic embryos. For optimisation of grape regeneration system the propagation of calli was attempted in Richter 110 cultivar, there was no any significant differences in the measured values, but CPE medium proved more successful in maintaining embryogenic capacity of callus. We experienced high developmental differences between the propagated embryogenic culture of Richter 110, Teleki 5C and Chardonnay derived from MSNOA liquid medium and from MSE solid medium. Regenerated plants from embryogenic callus were obtained in 21 genotypes, in Chardonnay cultivar CP medium influenced more positively the plant regeneration than the MS/2 medium.

Restricted access