Authors:M. Mardani-Talaee, A. Zibaee and V. Rahimi
Effects of six commercial tomato cultivars, Super Strain B, Super crystal, Hed rio grande, SUN 6108 f1, Rio grande UG and Cal.JN3 were determined on digestive physiology and life table parameters of Chrysodeixis chalcites (Esper). The highest values of consumed foods were determined in the larvae fed on Rio grande UG and Cal.JN3 while the lowest values were observed on SUN 6108 f1. Statistical difference was found in the pupal weight by the highest value on SUN 6108f1. The highest values of intrinsic rate of increase (rm) and the net reproductive rate (R0) were obtained on SUN 6108 f1 but the lowest values were obtained on Cal.JN3. Significant differences were also found in activities of digestive enzymes including specific proteases, α-amylase, glucosidases and TAG-lipase. Our findings showed that the highest and the lowest activities of specific proteases and TAG lipase were obtained on Rio grande UG and SUN 6108 f1, respectively. In addition, activities of the carbohydrases were the highest in the larvae fed on Cal.JN3. The demographical and physiological findings here revealed Cal.JN3 and Rio grande UG as the partially unsuitable cultivars for C. chalcites in comparison with other ones which may be recommended in integrated pest management.
Authors:H. Firouzbakht, A. Zibaee, H. Hoda and M. M. Sohani
Virulence of the two isolates of Beauveria bassiana, BB2 and AM-118, were evaluated on adults of a predatory hemipteran, Andrallus spinidens Fabricius by conidial bioassay and enzymatic activities. Results of the bioassay revealed LC50 of 37×104 and 15×103 spore/ml for isolates BB2 and AM-118, respectively. Activities of chitinase, lipase and ALP showed the higher activity in the media inoculated by AM-118 while no statistical differences were observed in activity of ACP. Although no statistical differences were found in general protease and Pr1 activities but activity of Pr2 in AM-118 was significantly higher than that of BB2. Activity of general esterases demonstrated no statistical differences when α- and β-naphtyl acetate were used as substrates but activity of glutathione S-transferase in AM-118 was higher than that of BB2 by using CDNB and DCNB as specific reagents. Results of the current study indicated higher virulence of isolate AM-118 against adults of A. spinidens by lower LC50 value and higher activities of the enzymes involved in pathogenicity. Recruiting of these isolates against C. suppressalis must be considered by their adaptability of A. spinidens. Moreover, AM-118 has been isolated from rice fields of northern Iran, so it may somehow indicate a type of host-microorganism interaction.