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Desirable wheat glycolipid routine analysis for the first time enables selection for a significant quality factor among bread quality hard wheat experimental cultivars. Prior to producing sufficient quantity of later generation experimental wheats to allow milling and baking, objective chemical testing of the protein and lipid components of the endosperm can make the wheat breeding process more efficient by earlier elimination of cultivars with inferior quality traits. Tandem mass spectrometric (MS—MS) analysis of digalactosyldiglycerides (DGDG) from total wheat endosperm lipid extract is now suitable as a routine desirable lipid ranking tool. This automated multistep technique eliminates time consuming fractionation, selective extraction, and chromatographic procedures of the past decades that resulted in limited capacity precluding extensive routine application.

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