Authors:B. Lanza, M.G. Di Serio, L. Giansante, G. Di Loreto and L. Di Giacinto
The quality of monovarietal extra virgin olive oil from cv. ‘Taggiasca’ is influenced by many factors that have impact on shelf-life as well as on sensory and healthy properties of the product. The aim of the work was to recreate the conditions similar to those in consumer sales point (conditions of “shelf”), maintaining the olive oil packaged in dark-green bottles at room temperature (between 18 and 25 °C) under artificial light and away from heat sources, monitoring the oils up to 12 months from bottling with quarterly sampling for the main chemical, physico-chemical, and sensory parameters related to the quality. After one year of storage, an organoleptic alteration with reduction of the attributes ‘fruity’, ‘pungent’, and ‘bitter’, as well as the occurrence of ‘rancid’ defect, was observed. This alteration was found to be accompanied by a decrease in phenolic substances and tocopherols and an increase in primary and secondary oxidation products. The composition of the volatile fraction showed a slight increase of substances related to rancid defect, a constant trend of compounds related to fruitiness, and a slight decrease in alcohols. It can be concluded that the optimum time of storage of the oil under the above-mentioned conditions is approximately 9 months.
Authors:J. D. Robertson, S. B. Crane, S. A. Wickline and G. M. Lanza
Angiogenesis is integral to the development and progression of atherosclerotic disease and solid tumor growth. New microvessels form in atherosclerotic plaque and the presence of new vessels has been associated with carotid plaque instability. Likewise, solid tumor growth depends upon angiogenesis to provide tumor cells with oxygen and nutrients. Recently, Lanza et al. have demonstrated molecular imaging of angiogenesis both in human melanoma xenografts in nude mice and atherosclerotic rabbits by magnetic resonance imaging (MRI) with clinical magnet strengths using ανβ3-targeted nanoparticles developed in their lab. ανβ3-integrin is a selective molecular epitope expressed by angiogenic endothelium and the MRI contrast agent consists of a lipid-encapsulated, liquid perfluorocarbon nanoparticle directly coupled to a selective ανβ3 ligand. The nanoparticle also contains the paramagnetic contrast agent gadolinium linked to the nanoparticle as Gd-DTPA-bis-oleate. In this work we report on the use of neutron activation analysis to confirm the Gd content of the nanoparticle formulations and determine the biodistribution of Gd post injection.