A number of nitrogen fixing bacteria has been isolated from forest phyllosphere on the basis of nitrogenase activity. Among them two best isolates are selected and identified as Corynebacterium sp. AN1 & Flavobacterium sp. TK2 able to reduce 88 and 132 n mol of acetylene (I08cells-1h-1) respectively. They were grown in large amount and sprayed on the phyllosphere of maize plants as a substitute for nitrogenous fertilizer. Marked improvements in growth and total nitrogen content of the plant have been observed by the application of these nitrogen-fixing bacteria. An average 30-37% increase in yield was obtained, which is nearer to chemical fertilizer treatment. Comparatively better effect was obtained by application of Flavobacterium sp.
Tannase an industrially important enzyme was produced by
DBF9 through a solid-state fermentation (SSF). The organism produced good amount of enzyme and gallic acid in wheat bran among the solid substrate used in SSF. Maximum enzyme and gallic acid production occurred in 5% tannic acid after 72 h. Eighty percent initial substrate moisture and 30 °C temperature was found suitable for tannase production.
Authors:D. Banerjee, S. Manna, S. Mahapatra, and B. Pati
Three medicinal plants
Ocimum sanctum, Ocimum bacilicum
were screened to study endophytic diversity of the plants. Altogether 103 fungal endophytes belonging to fourteen genera were isolated. Leaves of all three medicinal plants were colonized by a great number of endophytic fungi. Leaves of
were colonized by the most, that is, eleven endophytes. Highest Shannon-Wiener index (2.256) was exhibited by
with the highest Simpson’s diversity (0.8654) indicating great species specificity.
showed the highest similarity coefficient. Some fungal genera have been showed to be host specific. In the present study
sp. exclusively colonized
sp. colonized only