Authors:Tingting Zhang, Wanting Yin, Bo Jin, Tong Li and Chen Ma
A sensitive, stability-indicating reversed-phase high-performance liquid chromatography with diode array detection (HPLC–DAD) method has been developed for the determination of TBI-166 and its 10 kinds of related impurities. Chromatographic separation was achieved on a Kromasil ODS column (250 mm × 4.6 mm, 5 μm), with a gradient elution of the mobile phase system consisting of acetonitrile and 1% ammonium formate solution (with 0.2% formic acid). The flow rate was 1.0 mL/min, and the detection wavelength was set at 251 nm. The method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to selectivity, linearity, limits, accuracy, precision, and robustness. The calibration curves were linear from LOQ to 150% of the specification limit of impurity with correlation coefficients not less than 0.999. The limits of quantitation were between 0.123 and 0.257 μg/mL. Accuracy for the related substances was estimated by the recovery ranged from 94.6% to 111.2%. The method was proved to be reliable for the determination of related substances in TBI-166 bulk drug, which is essential and important in the quality control.
Authors:Wenlong Zhang, Xiaodan Liu, Mengcheng Liu, Bo Ma, Li Xu and Junwei Wang
Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.
Authors:Shao-Ning Yu, Zheng-Ping Fan, Li Ma, Yan-Zhao Yang and Bo-Rong Bao
The influence of the concentration of nitric, hydrochloric and phosphoric acids, petroleum sulfoxides (PSO), salting-out agent,
kind of diluent and temperature on the distribution ratio of U(VI) and Th(IV) has been systematically studied. It is found
that the extraction regularity of PSO is similar to that of TBP. The distribution ratio in phosphoric acid is lower, but it
increases with the increase of hydrochloric acid concentration and reaches a high value. The U(VI) exhibits the maximum distribution
ratio at 3–4 mol/l HNO3. The distribution ratio of U(VI) and Th(IV) increases rapidly in the presence of a salting out agent. The extracted compounds
are determined to be UO2(NO3)22PSO and Th(NO3)42PSO. The extraction enthalpies of U(VI) and Th(IV) with PSO were also calculated.
Authors:Yishan Wu, Yuntao Pan, Yuhua Zhang, Zheng Ma, Jingan Pang, Hong Guo, Bo Xu and Zhiqing Yang
This paper traces the history of China Scientific and Technical Papers and Citations database (CSTPC) since its founding in 1988. The fact that most Chinese scientists publish their research results in Chinese journals requires that China establish SCI counterparts dedicated to domestic S & T journals. The article describes the selection criteria for source journals, the approach used to adjust the structure of source journals, the criteria for selecting items to be included in the database, and the indexing method. Then it discusses the impact upon government R & D administration agencies and the science community in general by both CSTPC team and CSTPC database. Finally, the article analyzes the main factors that lead to the primary success of CSTPD. The authors encourages information workers in other non-English developing countries to build up similar databases.
Authors:Kang-Zhen Xu, Xian-Gang Zuo, Hang Zhang, Biao Yan, Jie Huang, Hai-Xia Ma, Bo-Zhou Wang and Feng-Qi Zhao
A new high-energy organic potassium salt, 1-amino-1-hydrazino-2,2-dinitroethylene potassium salt [K(AHDNE)], was synthesized by reacting of 1-amino-1-hydrazino-2,2-dinitroethylene (AHDNE) and potassium hydroxide in methanol aqueous solution. The thermal behavior of K(AHDNE) was studied using DSC and TG/DTG methods and can be divided into three obvious exothermic decomposition processes. The decomposition enthalpy, apparent activation energy and pre-exponential factor of the first decomposition process were −2662.5 J g−1, 185.2 kJ mol−1 and 1019.63 s−1, respectively. The critical temperature of thermal explosion of K(AHDNE) is 171.38 °C. The specific heat capacity of K(AHDNE) was determined using a micro-DSC method, and the molar heat capacity is 208.57 J mol−1 K−1 at 298.15 K. Adiabatic time-to-explosion of K(AHDNE) was also calculated. K(AHDNE) presents higher thermal stability than AHDNE.
Authors:Li Li, Wei Wang, Xiangshang Xu, Hui Wang, Shujie Liao, Wei Li, Weina Zhang, Dan Liu, Bo Cao, Shixuan Wang, Keng Shen and Ding Ma
Radioimmunotherapy (RIT) has emerged as an attractive and promising strategy for the management of malignant diseases. It
has been proven to be quite effective in the treatment of numerous tumors, such as non-Hodgkin lymphoma, metastatic prostate
cancer, melanoma, thyroid cancer, colon cancer and so on. The RIT currently used is mainly based on monoclonal antibodies
to recognize target antigens. As antibodies are large molecules, this method of RIT has some limitations in in vivo use, such
as the immunogenicity, the high costs and low efficiency of production. Aptamer is discovered and selected by SELEX technology.
As specific recognizers and binders, aptamers and antibodies have such a close similarity as to be interchangeable to some
extent. But, aptamers have many advantages over antibodies: higher affinity and specificity, smaller molecular weight, more
easily synthesized and modified, more rapidly penetrating into tumors, higher tumor-to-blood distribution ratio and more easily
to be cleared. In addition, since aptamer has almost no immunogenicity in vivo, it can be repeatedly administered. Thus, we
believe that aptamer-based RIT will be a feasible and promising way to treat human cancers, and it might display better results
in cancer treatment than antibody-based RIT. In conclusion, aptamer-based RIT is hopeful to become a key therapeutics in cancer
radiotherapy in the near future.