Authors:C. Riefolo, D. Ficco, L. Cattivelli, and P. Vita
The genetic variations of high and low molecular weight glutenin subunits (HMW-GS and LMW-GS) as well as of ω- and γ-gliadins in 562 accessions of 7 tetraploid Triticum turgidum L. subspecies were investigated using sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE). A total of 26 HMW-GS alleles (7 at Glu-A1 and 19 at Glu-B1 loci) with 63 allelic combinations, as well as 11 LMW-GS alleles (5 at Glu-A3, 4 at Glu-B3 and 2 at Glu-B2 loci) with 26 allelic combinations, were detected. Two novel HMW-GS, called B1cf and B1cg, were discovered in T. dicoccum, B1cg was also found in T. turanicum. The Glu-B1 locus showed the highest values of genetic diversity index (H), with a mean of 0.72. As regards gliadins, 8 alleles at Gli-B1 locus have been found. The dendrogram based on allelic frequencies, revealed that T. durum, T. carthlicum and T. polonicum grouped a part from the other subspecies. This behaviour suggested probably different evolutive pathways among the tetraploid wheats.