In this study, bromine (Br), chlorine (Cl), potassium (K) and sodium (Na) were measured in the blood of Brazilian inhabitants
from Southeast (S) and Northeast (N) regions. A reference range was established as a function of sex and considering lifestyle
factors (non-smokers and non- drinkers). For both regions lower values of K were found in females when compared to males and
lower values of Na were found in males compared to females. Increasing trends for Na and Br were observed in the Northeast
Concentrations of Br, Ca, Cl, K and Na in whole blood of dystrophin-deficient mouse [the Dmdmdx line] were determined using
NAA, resulting in reference values that are relevant for clinical blood investigation. The comparison with human being whole
blood values was also performed in order to establish possible indexes and similarities among the experimental and clinical
Authors:L. C. Oliveira, C. B. Zamboni, and J. Mesa
Neutron activation analysis, using Au as flux monitor, was applied to determine the concentrations of Br, Cl, K and Na in
blood of healthy male and female blood donors, selected from blood banks and hematological laboratories from different regions
of Brazil. The aims of this study were to collect more reference values of the Brazilian population as well as to perform
hematological investigations. The advantages as well as the limitations of using this nuclear procedure are discussed.
Authors:L. C. Oliveira, C. B. Zamboni, F. A. Genezini, A. M. G. Figueiredo, and G. S. Zahn
In this study we show that the NAA technique can be used to perform clinical analyses of blood and urine, with many advantages towards the conventional methods. From the knowledge of the neutron flux and the induced activity, the concentration ofelements were obtained. In comparison to the conventional techniques, this methodology uses smaller quantities of biological material and allows the simultaneous evaluation of the concentrations of several elements in biological samples at once, something not always possible in the conventional clinical analysis. Another important advantage is that it eliminates the use of standard materials, thus making the analyzing process practical and economic.
Authors:C. B. Zamboni, L. C. Oliveira, L. Dalaqua Jr, and J. Mesa
Neutron activation analysis technique, using Au as flux monitor, was applied to determine element concentrations of Ca, K,
Na, Mg, Mn and Sr in certified reference material (NIST 1400 Bone Ash). The results were compared with those using comparative
INAA and they were compatible. The same results were obtained using the recommended k0 factors, in order to obtain the activation cross section as input in concentration for the same reference material. Some
applications in the health area aiming clinical evaluation in bone samples of medium and small-sized animals were performed
and the viability of using this methodology was discussed.
Authors:C. Zamboni, M. Suzuki, S. Metairon, M. Carvalho, and O. Sant’Anna
The Br (0.0022 ± 0.0006 gL−1), Ca (0.113 ± 0.012 gL−1), Cl (3.07 ± 0.36 gL−1), K (2.63 ± 0.14 gL−1), Mg (0.045 ± 0.002 gL−1) and Na (2.09 ± 0.10 gL−1) concentrations were determined in whole blood of SJL/J mice using the Neutron Activation Analysis (NAA) technique. Eleven
whole blood samples were analyzed in the IEA-R1 nuclear reactor at IPEN (São Paulo, Brazil). These data contribute for applications
in veterinary medicine related to biochemistry analyses using whole blood. Moreover, the correlation with human blood estimation
allows to checking the similarities for studying muscular dystrophy using this model animal.
Authors:L. Kovacs, C. Zamboni, L. Oliveira, V. Salvador, I. Sato, and M. Azevedo
The concentrations of Cl, K and Na were analyzed in human body fluids (whole blood and serum) using NAA, resulting in the
first biochemical baseline values for the Brazilian population. These data permitted us a discussion about the advantages
and limitations of using this analytical technique for clinical chemistry.
Authors:C. Zamboni, S. Metairon, M. Suzuki, M. Furtado, O. Sant’Anna, and D. Tambourgi
Mice genetically selected for high antibody responsiveness (HIII) were immunized against different Bothrops species snake venoms from distinct region of Brazil. The Neutron Activation Analysis technique was used to evaluate the whole
blood concentrations of elements of clinical relevance [Ca, Cl, K, Mg and Na] in order to establish a potential correlation
between antibody response and blood constituents after Bothrops venom administration for clinical screening of envenomed patients.
Authors:S. Metairon, C. Zamboni, L. Kovacs, F. Genezini, N. Santos, and E. Vilela
Neutron activation analysis has been used to determine Br, Ca, Cl, K, Mg and Na concentrations in whole blood of patients
with chronic kidney disease (CKD) as well as in whole blood of normal individuals (control group). The dependence of the elements
concentration in function of sex, age, time and type of treatment were investigated. The similarities and differences between
healthy individuals and CKD are discussed.
Authors:J. D. T. Arruda-Neto, A. C. Cestari, C. B. Zamboni, M. Saiki, G. P. Nogueira, L. E. C. Fonseca, M. V. Manso-Guevara, A. Deppman, V. P. Likhachev, J. Mesa, O. A. M. Helene, S. A. C. Jorge, M. N. Martins, A. N. Gouveia, O. Rodriguez, F. Guzmán, and F. Garcia
Neutron activation analysis has been used to study uranium incorporation in poultry bones as function of chow doped with: (a) uranium (20 ppm); (b) U-doped food (20 ppm) plus phytase (120 ppm) and (c) U-doped food (20 ppm) plus phytase (180 ppm). To investigate this situation experiments involving several groups of Cobb broilers was performed. Two animals per group were sacrificed weekly up to their adultness and uranium concentration in the tibia was measured. It was observed that the concentration of uranium (µg U/g bone) is decreasing all along the animal life spanning period of 14-42 days. This behavior suggests that the skeleton mass is growing faster than the corresponding accumulation of uranium. The administration of phytase seems not to alter this scenario.