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Abstract  

Radiometric titrations are reported based on the formation of poorly soluble chelate complexes of 8-hydroxyquinoline and its derivatives with calcium ions. Calcium chloride reagent solution labelled with45Ca was used for the titrations. The slightly alkaline medium used in the titrations made complete the solution of the substances and the formation of chelate complexes. The appropriate conditions of the titrations of the various compounds have been determined. The radiometric titrations were carried out in an apparatus suitable for the detection of soft β-emitting isotopes.

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Abstract  

The nuclear track technique (NTT) is used to enhance the porosity of silica micro-particles. The enhanced porosity is a result of the formation of surface and interior pores or tracks in the silica by the action of external and internal fission fragments. The fission tracks produced at the surface and within the interior of the micro-particles are a result of coating the particles with trace quantitities of uranium, instead of having trace quantities of uranium incorporated within the silica matrix.

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Acta Biologica Hungarica
Authors:
Á. Horváth
,
P. Sántha
,
V. Horváth
,
Nóra Török
,
I. Nagy
,
G. Jancsó
,
Cs. Vágvölgyi
, and
F. Somogyvári

A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.

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Winter wheat (Triticum aestivum L.) yield samples taken from a consecutive series of crop years representing drought, normal and moist conditions at the Nagygombos experimental site of the Szent István University have been evaluated. The impact of N topdressing and crop year conditions on the performance of protein and wet gluten content were studied.The results suggest that ascending levels of N topdressing, and increased number of applications had a beneficial effect on the protein content, as well as on wet gluten values. Crop year had a significant effect on quality manifestation. The drier the crop year, the better the recorded quality performance. The magnitude of changes was bigger in case of gluten than in that of protein values. There were minor differences between the two varieties examined, especially in their response patterns to nitrogen supply. Dough formation depends mainly on the gliadin/ glutenin ratio, but there is less information on the agronomic and climatic impacts that may cause alterations in that.

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Journal of Thermal Analysis and Calorimetry
Authors:
G. Bognár
,
Cs. Pintér
,
B. Horváth
,
T. Sydo
,
E. Ligeti
,
J. Pulai
, and
D. Lőrinczy

Abstract  

Osteonecrosis (ON) of the femoral frequently occurs after steroid medication. One of the final pathways leading to steroid induced ON is thought to be pathologic fat metabolism. The pathobiological mechanism underlying the induction of fat metabolism outslides by steroids leading to ON has not been fully elucidated. The purpose of this study was to examine the intraoperative obtained gluteal fat tissue from ON patients with histology, gas chromatography (GC) and differential scanning calorimetry (DSC) and to compare them with otherwise healthy patient’s samples. The histological sections showed no significant differences compared with the control group. GC revealed that fraction of saturated fatty acids decreased in ON samples from mean values of controls of 24% to 21, the polyunsaturated fraction from 20 to 14%. The monounsaturated acids showed an increase from mean rate of 52% of the controls to 65% of steroid treated samples. DSC curves correlate with chromatographic analysis of the tissue fatty acids (Steroid treated, heating between 0–100°C: T m=5.7°C, ΔH= −15.8J/g−1; heating between −20–100°C: Tm= −9.96 and 5.85°C, ΔH= −59.17 and −16.2 J g−1. Non-necrotic, heating between 0–100°C: two separable transition with Tm=5.7 and 9.9°C, total ΔH= −20.8 J g−1; heating between −20–100°C: Tm= −10.9 and 4.95°C, total ΔH= −75.8 J g−1.) Our preliminary findings are rather tendentious. Further investigations are needed with higher sample rate and under other anamnestic circumstances too.

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Journal of Thermal Analysis and Calorimetry
Authors:
Cs. Pintér
,
G. Bognár
,
B. Horváth
,
T. Sydo
,
E. Ligeti
,
J. Pulai
, and
D. Lőrinczy

Abstract  

Avascular necrosis of human femoral head (ANFH) causes incongruity in the joint that leads to disability in patients requires total hip arthroplasty (THA). Several etiological factors of ANFH have been proposed in the literature but there are cases of idiopathic origin. We observed macroscopic variation in quality of the subcutaneous fat tissue in patients with ANFH compared to patients with osteoarthritis or hip fracture during THA procedures. The samples were analysed by histology, gas chromatography (GC) and differential scanning calorimetry (DSC). Conclusion: the alteration in the fatty acid profile did not cause histological changes, however we could detect biochemical changes using DSC and GC.

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Journal of Thermal Analysis and Calorimetry
Authors:
Cs. Pintér
,
G. Bognár
,
B. Horváth
,
T. Sydo
,
E. Ligeti
,
J. Pulai
, and
D. Lőrinczy

Abstract  

The osteonecrosis or avascular necrosis of the antero-superior part of the human femoral head (ANFH) often causes incongruity in the hip joint and leads to severe pain and disability in middle aged patients. This abnormality often requires surgical intervention, mainly total hip arthroplasty (THA). The possible pathomechanisms of ANFH are fat embolism, arterial occlusion, fatty necrosis of osteocytes and intraosseous hypertension. Factors have been proposed in the literature that can lead to this condition are alcohol abuse, steroid therapy, metabolic changes, dyslipidaemia. In some case we can not verify any factor; these are the so called idiopathic ANFH cases. We observed macroscopic variation in colour and consistency of the subcutaneous fat tissue in patients with ANFH compared to osteoarthritis or hip fracture during THA procedures. Subcutaneous fat tissues during THA from a patient with ANFH due to alcohol abuse were compared with an otherwise healthy patient who underwent surgery due to traumatic hip fracture. No histological changes were notified in the size; shape of adipocytes and in the cells of the septae of the connective tissue. Gas chromatography showed that the patient with alcoholic ANFH had less long chain fatty acids. DSC revealed, that in case of non-necrotic sample as a reference, during heating between 0–100°C two separable transitions are with T m=5.7 and 9.9°C, total ΔH= −20.8 J g−1. In −20–100°C range endotherms with T m= −10.9 and 4.95°C, total ΔH= −75.8 J g−1 could be detected. In case of alcohol-induced avascular necrosis we have found endotherms between 0–100°C with: T m=7.3°C, total ΔH= −26.9 J g−1, and heating between −20–100°C: T m= −0.25°C, total ΔH= −103.3 J g−1 thermal parameters. The alteration in the fatty acid profile did not cause histological changes, but we were able to detect it with analytical methods e.g. DSC and gas chromatography.

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Abstract  

A frequently used method in determining the radium concentration of water is the radon emanation method. When radon gas is transferred to the Lucas-cell usually CaCl2 is used to remove the water traces. When we measured the background of the system using ultra clear distilled water the results were astonishing. The detailed investigation has shown that the unwanted radon originated from the CaCl2, contained about 1000 Bq/kg of 226Ra. Depending on the time interval between two measurement, the radon deriving from the CaCl2 disturbed the measurements.

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Genetic effects that contribute to the risk of developing chronic obstructive pulmonary disease (COPD) have been reported. Our purpose was to estimate the possible genetic influence on CT features related to COPD in twins.

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Acta Biologica Hungarica
Authors:
E. Horváth
,
G. Papp
,
Z. Gazdag
,
J. Belágyi
,
Á. Blaskó
,
J. Deli
,
Cs. Vágvölgyi
, and
M. Pesti

A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

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