The recent review summarizes the major achievements in discovery of role of phytoglobins in mediation of nitric oxide generated cellular functions in higher plants. Genes encoding non-symbiotic hemoglobins have been cloned from several plant species. The expression pattern of these genes show tissue-specificity that is also under the control of stress factors like hypoxia. The nitric oxide has pivotal role in signalling pathway specifically in hypersensitive reactions and programmed cell death. Production of transgenic tobacco plants overexpressing the alfalfa hemoglobin showed altered necrotic symptoms after treatment with nitric oxide generating compounds or infection by necrotic pathogens. The present review helps to outline the similar relation between hemoglobin and nitric oxide in plants as it was found in animal cells.