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A history of having substantial Chlamydia trachomatis exposure as detected by serum antibodies is a cofactor of human papillomavirus (HPV) mediated cervical carcinogenesis. In this study, we examined the concurrent C. trachomatis infections in cytologic atypia of the uterine cervix in order to evaluate the impact of C. trachomatis infection in patients with high risk for cervical intraepithelial neoplasia. Cervical scrapes form 707 patients were subjected to PCR amplification with primer sets for HPV and C. trachomatis . Based on negative beta-globin results, 10 specimens were not eligible for further analysis. Oncogenic HPV types were detected in 278 specimens (39.8%). C. trachomatis was found only in six specimens (0.9%). In conclusion, concurrent C. trachomatis infection was uncommon and hence it was an improbable risk factor in cytologic atypia.

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Acta Botanica Hungarica
Authors: E. Farkas, Á. Kiss, G. Fehér, B. Bolla, and E. Farkas
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Soy is increasingly used as a food additive. In women, it is recommended as an alternative to hormonal replacement therapy and/or a preventive agent against breast cancer and osteoporosis. Previous data revealed that rats fed on raw soybean diet developed pancreas hypertrophy and hyperplasia. An animal model was used in our experiment to examine the effects of raw soybean on parotid gland of rats. The purpose of this study was to light on the role of different neuropeptide-containing nerve fibres on changes in the acinar cells. The morphological structure and the neuropeptide-containing nerve fibers (NPY, GAL, SOM, SP, CGRP, VIP) of the glands were examined by light and electronmicroscopy. Significant increase of the organ weight was detected in the animals fed by raw soybean compared to control samples. Changes in the number of different neuropeptide-containing nerve fibres were various: Significant decrease in the NPY-immunoreactive (IR) and significant increase in the GAL-IR nerve fibres were observed. Slight but not significant increase in VIP-IR; and no changes in the other IR nerve fibres were found. The electronmicroscopic alterations of acinar cells were manifest, where a large number of undifferentiated glandular cells were seen among the acini. Some of these cells contained two nuclei and their cytoplasm contained only a few secretory granules. These granules were similar to those in the mucous cells but not to the serous ones. The results presented here provide direct morphological evidence for the role of raw soy on the density of different neuropeptide-containing nerve fibres inducing proliferation in the acinar cells of parotid glands from rats. It is suggested that the hypertrophic changes in the glands might be caused by the alterations of nerve fibres.

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Aims

Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical–chemical technique that can be routinely performed with minimum damage.

Methods

Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle’s tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla’s hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 µL of sodium arsenite solution (50 µM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient.

Results and conclusion

All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

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Book reviews

Editor: K. T. KISS

Acta Botanica Hungarica
Authors: B. Bolla, L. Lőkös, G. Fehér, T. Pelyhe, and E. Farkas
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The interactions between surfactant and water were studied thermoanalytically focusing on the lyotropic liquid crystalline and microemulsion region in four ternary systems containing Cremophor EL and Cremophor RH40 as surfactants, neutral oil and isopropyl myristate as oily components. Subzero temperature DSC (SZT-DSC) measurements were carried out to determine the quantity of the bound water forming a hydration layer in surfactant microstructures, and the amount of free water, which has physico-chemical properties not much different from those of pure water. The variation of the surfactant:bound water ratio in the function of water concentration was also investigated. Phase changes detected by the SZT-DSC measurements were confirmed by polarization-microscopic and rheological investigations.

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Abstract  

The migration of HTO from a point source was studied in the soil of the storage of radioactive waste at horizontal distances of 10 to 40 cm from the source between 2 and 4 m depths at 5 different rainfalls, up to 7641/m2. The water movement changed from 0.17 cm/l (at 10 cm) to 0.28 cm/l (at 40 cm distance) when 186 1 was irrigated, while at 764 1 rainfall it was found to be 0.11 cm/l at every distance. The estimated parameters of a three-dimensional migration model constructed to characterize HTO movement revealed that the HTO distribution migrates downwards in a small, about 1 m thick layer with an initial rate of 0.17 cm/l to slow down to about 0.05 cm/l after 50 years. The distribution is spreading horizontally with a constant rate of about 0.08 cm/l.

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Lingual components of the autonomic nervous system are considered to be the most rostral portion of the enteric nervous system. Therefore our aim was to study the intrinsic nerve cell bodies and synapses using immunohisto-, immunocytochemical methods. Several small groups of ganglia with cell bodies immunoreactive (IR) for vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and substance P (SP) were observed just below the gustatory epithelium. A few somatostatin and galanin IR nerve cell bodies were also found. Many IR cell bodies were also demonstrated in the glands and next to blood vessels. Some of these cell bodies were multipolar and some of them were small neurons with an ovoid shape having only one process. Cell bodies positive for calcitonin gene-related peptide (CGRP) were detected neither in the superficial nor in the deep portion. Electronmicroscopical analysis demonstrated different IR nerve fibres having axo-somatic and axo-dendritic synapses with other immunonegative cells. In a few cases VIP IR nerve processes were found to synaptize with other VIP positive nerve cell bodies. These results support the existance of intralingual reflex in the tongue, where the ganglia might have an integrative role of the different neuropeptide containing nerve fibres.

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Acta Alimentaria
Authors: A. Blázovics, E. Fehér, I. Kocsis, E. Rapavi, E. Székely, L. Váli, and K. Szentmihályi

In general, biologically active polyphenolic compounds have indirect lipid lowering effects, antioxidant properties and they can also eliminate the accumulated toxic metal elements in the liver in cases of hyperlipidemy. Because of all these, we studied the effect of Beiqishen tea on altered lipid metabolisms, redox parameters and on liver metal contents. Although it was expected, there was not any liver protecting activity of the Beiqishen tea extract in the hyperlipidemic rat model, which was proved by biochemical and morphological studies. The reason for our expectation was the significant polyphenol content of Beiqishen tea. Although, the detoxifying activity of tea polyphenols could be observed in hyperlipidemy, at the same time toxic metal element content (As, Mo, Ni, Pb and Cr) of the tea infusion accumulated in the liver in both normo- and hyperlipidemic rats.

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In this study molecular markers linked to the Ry sto gene, which originates from the wild potato species Solanum stoloniferum and confers extreme resistance against PVY, were identified and the applicability of recently published Ry sto markers was analyzed. Three RAPD markers covering a total distance of 8.60 cM were detected in this experiment. The closest of these markers was located 0.53 cM from the gene. From among the published markers only one had diagnostic value in the experimental plant material, and mapped 2.95 cM from the gene, on the side opposite the RAPD markers developed in the present study. All the markers analyzed were present in Solanum stoloniferum accessions, irrespective of their resistance, indicating that these sequences are linked to the locus and not exclusively to the dominant allele of the Ry sto gene in the wild species. The inapplicability of several published markers indicates that the genetic background is decisive in this tetraploid and highly heterozygous species. This means that it may be necessary to develop markers from the breeding material itself, until the resistance gene is not cloned and cannot be used as a selection marker in marker-assisted selection.

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