Sertraline is an antidepressant drug. Sertraline was labeled with 131I by using iodogen method. Labeling yield was 85–90% and specific activity was approximately 64.75 GBq/mmol. The purification of radioiodinated Sertraline was performed by Sep Pak C-18 plus and the radiochemical purity was determined to be over 99%. Biodistribution studies were carried out by male Albino Wistar rats. The percentage of injected radioactivity per gram of tissue was calculated, and these data versus time curves were generated for organs and brain regions. The results showed that 131I labeled Sertraline may be a promising radiopharmaceutical for the investigation of serotonin 5-HT receptor functions of brain.
Chlordiazepoxide (CDZ) was labeled with 131I and investigated its radiopharmaceutical potential as a benzodiazepine receptor agent. Iodogen was used as iodination agent. Labeling yields were determined by radio-ITLC (instant thin layer chromatography) and paper electrophoresis. Labeling yield was approximately 90%. Purification was performed by Sep-Pak C-18 plus. Biodistribution studies were carried out by albino Wistar rats. 131I labeled chlordiazepoxide which is the 62.2 GBq/mmol specific activity was administered by tail vein into the rats. Rats were sacrificed by ether narcotization at certain time intervals and the organs were removed. The brains were dissected into their components. Their activities were counted by a gamma-counter. The activity per gram tissue was calculated and time–activity curves were generated.
Authors:H. Enginar, P. Ünak, F. Yurt, and F. Zümrüt Biber
An oestrogen derivative 3,17-a-oestradiolyl propyl 1,4,8,11-tetraazacyclotetradecanyl-1-(4-methylbenzoic acid)ester (ESTCPTA) that is 3,17-a-oestradiolyl propinol coupled to 1-(4-methylbenzoic acid)1,4,8,11-tetraazacyclotetradecane (CPTA) was synthesized in five steps. The product was purified by recrystallization in ethyl alcohol, and analysed by NMR and IR spectroscopy. ESTCPTA was labeled with 99mTc and radio thin layer chromatography (RTLC) and radio-paper electrophoresis were used to determine the radiochemical yields. Specific activity was approximately 23.7 GBq/mmol and the labeling yield was over 95%. The biodistribution studies were performed on female Albino Wistar rats. The rats were sacricified by ether narcotization at certain time intervals and the activity of the organs was counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generated.
Authors:P. Ünak, F. Yurt Lambrecht, F. Biber, and S. Darcan
This study was designed to evaluate iodine concentrations in drinking water samples using isotope dilution analysis (IDA)
in the Aegean region of Turkey A total of 76 drinking water samples from rural and urban areas in regional cities were analyzed.
The mean iodine concentration was 78±27 μg/l and iodine concentration ranges were within 69±26 μg/l and 103±6 μg/l.
Authors:H. Enginar, P. Unak, F. Yurt Lambrecht, and F. Biber
An estrogen derivative 1-(3, 17-α-estradiolyl propin-1-yl-3-(1,4,8,11-tetraazacyclotetradecyl)-propanate (ESTACPA) was synthesized.
The product was purified by HPLC and characterized by NMR and IR spectroscopy. The synthesized compound was labeled with 99mTc. The biodistribution studies were performed on female Albino Wistar rats. The rats were sacrificed and their organs were
removed. The radioactivities of the organs were counted using a gamma-counter. The activity per gram tissue was calculated
and time versus activity curves were generated. The 99mTc-ESTACPA uptake by the uterus and ovary such as ER-rich tissues, were observed. The pancreas and stomach also showed a significant
Authors:P. Unak, F. Lambrecht, F. Biber, İ. Medine, and S. Teksoz
Famotidine, a gastrointestinal drug was labeled with 99mTc and its radiopharmaceutical potential was observed on male Albino Wistar rats. Labeling yield was over 95%. Average specific
activity and n-octanol/water partition coefficient (lipophilicity) were approximately 74 MBq/mg-0.66 GBq/mg and 3.4, respectively.
Biodistribution studies were performed on Albino Wistar rats. The activity per gram tissue was calculated and time-activity
curves were generated. The majority of the activity was observed in stomach, small intestines and kidneys. Liver and kidneys
showed lower uptake compared to other H2-receptor rich tissues. Results show that 99mTc-famotidine is H2receptor specific.
Somatostatin inhibits the release of growth hormone (somatotropin) from the Anterior Pituitary. The main use of derivatives
of somatostatin is to diagnose growth hormone problems and to use against some forms of cancer which involve growth hormone.
Also somatostatin suppresses gastric acid secretion, gallbladder contractions, and pancreatic enzyme secretion. The aim of
the current study is to investigate new bifunctional agents for labeling with 99mTc. Therefore D-Penicillamine was used as a bifunctional agent and compared to DTPA in the labeling with 99mTc. Quality controls were established using thin layer radio chromatography (TLRC) and electrophoresis techniques. In addition,
high performance liquid radio chromatography (HPLRC) was also performed for elimination of possible uncertainties. The radiolabeled
complexes maintained their stabilities throughout the study. The results obtained showed that 99mTc-D-Penicillamine-somatostatin is a promising potential radiopharm aceutical and an alternative of 99mTc-DTPA-somatostatin for in vivo and in vitro applications.
Iodide traces in common salts consumed in Turkey have been determined by isotope dilution analysis. Iodide was precipitated
by stoichiometric amount of AgNO3. Iodide-131 was used as tracer. Electrophoresis was performed to separate Ag131I from excess131I−. Zone of Ag131I was cut off electrophoresis paper and counted with a NaI(Tl) scintillation counter. Count rates were plotted versus added
KI concentrations. Unknown iodide amounts were found by using these linear plots. Iodide concentrations found in analyzed
salts were 9–58 μg/g.
The most suitable way of determination iodine-deficiency is to measure iodine concentrations in water and urine. For this
reason, a method that can determine iodide concentrations in drinking water and suitable for routine analysis, is developed.
Water samples have been collected from four Aegean localities: Izmir, Salihli, Ödemis and Tire situated in the western Turkey.
The method is based on substochiometric isotope dilution analysis. Iodile concentrations vary within 9.86–85.14 μg/l ranges
in the analyzed samples. Mean value is 44.92±22.07 μg/l.