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Regular disease monitoring is currently carried out in the most important Italian wheat growing areas. In 2007–08 stem rust was absent in all locations tested except Montelibretti (Rome, Central Italy), where two common wheat varieties “Arsenal” and “Compair” had stem rust infections. Two stem rust pathotypes were identified by testing in the greenhouse a set of differential lines/varieties carrying known genes for resistance to Puccinia graminis. These pathotypes corresponded to races MSK and PTK on the basis of the North American classification system. Genes Sr24 and Sr25 (both derived from Thinopyrum ponticum) and Sr31 (from Secale cereale), were resistant to the Italian pathotypes, and the lines carrying Sr38 (from Triticum ventricosum) were susceptible.Tests were carried out to determine the seedling stem rust response of durum and common wheat cultivars grown in Italy. Many durum wheat genotypes were resistant to MSK and PTK, while several common wheats were susceptible. The different response of the two species could be due to the source (common wheat) of stem rust inoculum. Molecular PCR markers, linked to Sr24, Sr25, Sr31 and Sr38, were used to detect their presence/absence in the genetic background of the durum and common wheat cultivars. The presence of Sr31 was shown in only two common wheat cultivars (“Colledoro” and “Sollario”), while several genotypes carried Sr38. No common wheat genotype was positive (to PCR analysis) for the presence of Sr24 or Sr25 genes, whereas no durum wheat was positive for the presence of Sr24, Sr25, Sr31 or Sr38.

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Summary  

Within the radiation protection physical surveillance program of some workers, involved in the decommissioning operations of a nuclear reprocessing plant of the Italian National Agency for New Technologies Energy and Environment, CR Saluggia (ENEA) in the north of Italy, some radiotoxicological analyses, on human urines, have been carried out, in order to determine the possible internal contamination of 241Am. After an in-depth study of a former extraction chromatographic method, based on the use of Microthene-722 supporting HDEHP, a more suitable method has been developed and adopted in our laboratory, based on the use of TRU-resin. This paper reports the results obtained with these two procedures as well as a comparison between them.

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Abstract  

Many biological samples (urines and faeces) have been analyzed by means of chromatographic extraction columns, utilizing two different resins (AG 1-X2 resin chloride and TRU), in order to detect the possible internal contamination of 239+240Pu and 241Am for some workers of a reprocessing nuclear plant in the decommissioning phase. The results obtained show on one hand the great suitability of the first resin for the determination of plutonium, and on the other, the great selectivity of the second one for the determination of americium.

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