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Abstract  

Both rapid and simple method of131I labelled MIBG was described. The labelling efficiency is more than 97% using Cu(Ac)2 as a catalyst, acetic acid, alcohol system as a medium of exchange reactions under the certain labelling conditions. The stability of the labelled131I-MIBG at the room temperature met the demand of the clinical application.

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Abstract  

The behavior of153Sm-EDTMP in vitro and vivo is analyzed by the size exclusion HPLC. The experimental results show that EDTMP amounts have an obvious effect on the stability in vitro and uptake of153Sm-EDTMP in the liver. HPLC analysis of urine sample indicates that153Sm-EDTMP es excreted in the original form. The behavior in vivo of153Sm-EDTMP containing 4 μg is similar to that of153Sm-EDTMP containing 50 μg EDTMP at 1 h post-injection.

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Abstract  

DTPA-Octreotide(Pentetreotide), a somatostatin analogue which can bind specifically and with high affinity to somatostatin receptor in vitro and vivo, labeled with99mTc by tin reduction in acetate buffer, has been characterized by Reverse-phase High performance Liquid Chromatography. The effect of different solvents, mobile phase pH, linear gradient and the injected volume on the separation efficiency was evaluated. The results show that the separation efficiency is best using μBondapak-C18 (300×3.9 mm2), linear gradient of 40% to 80% methanol (1.0 ml/min) in 0.05M acetate buffer (pH 5.5) over a 30 min period and maintaining for another 10 min. The labeled product is a mixture which mainly consists of five components (a, b, c, d, e) successfully proved by HPLC. Paper chromatography is also evaluated in this paper. It may be used to determine the radiochemical purity of the labeling product, but is not a good choice for the verification each components.

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Abstract

A novel acrylonitrile-capped poly(propylene imine) dendrimer (PAN4) was synthesized and characterized with FTIR and 1H-NMR. PAN4 and its precursor (poly(propylene imine) dendrimer (1.0GPPI) were employed to cure bisphenol A epoxy resin (DGEBA), and the nonisothermal reaction kinetics of DGEBA/PAN4 and DGEBA/1.0GPPI was systematically investigated using a differential scanning calorimeter (DSC) in a comparative way. The apparent activation energies determined with the Kissinger method were 59.7 kJ/mol for DGEBA/1.0GPPI and 53.9 kJ/mol for DGEBA/PAN4. Applied the Málek method, it was found that a two-parameter autocatalytic model (SB(m, n)) could well simulate the reaction rates, and further analysis of the reaction rate constants showed PAN4 could cure DGEBA at a greatly decreased rate by a factor a more than ten compared with 1.0GPPI control.

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Abstract  

The sorption of Eu(III) on calcareous soil as a function of pH, humic acid (HA), temperature and foreign ions was investigated under ambient conditions. Eu(III) sorption on soil was strongly pH dependent in the observed pH range. The effect of ionic strength was significant at pH < 7, and not obvious at pH > 8. The type of salt cation used had no visible influence on Eu(III) uptake on soil, however at low pH values, the influence of anions was following the order: Cl ≈ NO3  > ClO4 . In the presence of HA, the sorption edge obviously shifted about two pH units to the lower pH, whilst in range of pH 6–7, the sorption of Eu(III) decreased with increasing pH because a considerable amount of Eu(III) was present as humate complexes in aqueous phase, then increased again at pH > 11. The results indicated that the sorption of Eu(III) on soil mainly formed outer-sphere complexes and/or ion exchange below pH ~7; whereas inner-sphere complexes and precipitation of Eu(OH)3(s) may play main role above pH ~8.

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Acta Biologica Hungarica
Authors: Xiang-Rong Xu, Fu-Qing Tan, Jun-Quan Zhu, Ting Ye, Chun-Lin Wang, Yi-Feng Zhu, Hans-Uwe Dahms, Fan Jin and Wan-Xi Yang

We used single-cell gel electrophoresis (SCGE) to detect the integrity of sperm DNA of the teleost large yellow croaker, Pseudosciaena crocea, cryopreserved with Cortland solution and a range of 5% to 30% DMSO concentrations in order to test how sperm cryopreservation affected the DNA stability of nuclei. Electrophoresis was conducted for 60 min at 130 mA and 15 V. The comet images were analyzed with software CometScore 1.5, and parameters such as comet length, tail length and percentage DNA in the tail were obtained. Then the comet rate and damage coefficient were calculated. Results demonstrated that there were no significant differences in motility, comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%, 10%, 15% and 20% DMSO, while the sperm cryopreserved with 25% and 30% DMSO had a lower motility, higher comet length and damage coefficients than those of fresh sperm. There was a positive correlation between comet rate of cryopreserved sperm and the concentration of DMSO. Our results demonstrate that toxicity of the cryoprotectant is the main cause of DNA damage in cryopreserved sperm nuclei.

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Abstract  

Interfacial tension and interfacial adsorption parameters for benzyloctadecyldimethyl ammonium chloride (BODMAC) in three organic diluents were determined and interpreted. The interfacial activity of BODMAC is affected by the type of the organic diluent and the composition of the aqueous phase. The general order of interfacial activity of BODMAC is n-heptane (5% isobutanol) > carbon tetrachloride > chloroform. The effectiveness of adsorption of BODMAC is not only dependent on the organic diluent, but also on the inorganic electrolytes in the aqueous phase.

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