Authors:G. Bognár, I. Szabó, Cs. Pintér, E. Ligeti, and D. Lőrinczy
Long head of the biceps (LHB) is an intra-articular tendon component of the shoulder joint. The function of this tendon is
complex. First, it is an origin of flexion in upper limb, and second it plays role in joint stabilisation during shoulder
movements. Histological type of tendon tissues is connective tissue. The mechanical behaviour of connective tissue is primarily
determined by the composition and organisation of collagens. In tendons, type I collagen is the principal structural element
of the extracellular matrix, which acts to transmit force between bones or bone and muscle. Owing to the special localisation
of this tendon, the intra-articular mechanical forces affect it to a considerable extent. The LHB is known as a source of
pain in pathologic states of the shoulder joint. The goal of this study was to establish the calorimetric standards of the
LHB in different ages, and to observe the changes of thermal properties of collagen during lifetime. LHB samples were taken
from 38 cadavers (between ages 0 and 90 years) without macroscopic sign of shoulder pathology. DSC analyses were performed
with SETARAM Micro DSC-II. The thermal denaturation parameters varied between Tm (°C): 57, ΔH (J/g): 0.26 (age: 0 year) and Tm (°C): 62.92, ΔH (J/g): 1.28 (age: 90 years). The ageing of collagenous tendon tissue can be clearly followed in changes of thermal denaturation
properties. The knowledge of the ageing of normal collagen provides a good basis to analyse further the LHB pathology.
Authors:D. Bódizs, S. Dési, G. Keömley, and T. Pintér
A semi-automated instrumental gamma-spectrometric measuring system with a sample changer has been developed for determination of the radioactive fission products coming from different parts of the primary coolant circuit. The measuring geometry assures higher sensitivities under normal operation conditions and lower ones for significant fuel cladding failures. The minimal detection limits are in the range of some ten Bq/l.
Authors:Cs. Pintér, G. Bognár, B. Horváth, T. Sydo, E. Ligeti, J. Pulai, and D. Lőrinczy
The osteonecrosis or avascular necrosis of the antero-superior part of the human femoral head (ANFH) often causes incongruity
in the hip joint and leads to severe pain and disability in middle aged patients. This abnormality often requires surgical
intervention, mainly total hip arthroplasty (THA). The possible pathomechanisms of ANFH are fat embolism, arterial occlusion,
fatty necrosis of osteocytes and intraosseous hypertension. Factors have been proposed in the literature that can lead to
this condition are alcohol abuse, steroid therapy, metabolic changes, dyslipidaemia. In some case we can not verify any factor;
these are the so called idiopathic ANFH cases.
We observed macroscopic variation in colour and consistency of the subcutaneous fat tissue in patients with ANFH compared
to osteoarthritis or hip fracture during THA procedures. Subcutaneous fat tissues during THA from a patient with ANFH due
to alcohol abuse were compared with an otherwise healthy patient who underwent surgery due to traumatic hip fracture. No histological
changes were notified in the size; shape of adipocytes and in the cells of the septae of the connective tissue. Gas chromatography
showed that the patient with alcoholic ANFH had less long chain fatty acids. DSC revealed, that in case of non-necrotic sample
as a reference, during heating between 0–100°C two separable transitions are with Tm=5.7 and 9.9°C, total ΔH= −20.8 J g−1. In −20–100°C range endotherms with Tm= −10.9 and 4.95°C, total ΔH= −75.8 J g−1 could be detected. In case of alcohol-induced avascular necrosis we have found endotherms between 0–100°C with: Tm=7.3°C, total ΔH= −26.9 J g−1, and heating between −20–100°C: Tm= −0.25°C, total ΔH= −103.3 J g−1 thermal parameters. The alteration in the fatty acid profile did not cause histological changes, but we were able to detect
it with analytical methods e.g. DSC and gas chromatography.
Authors:S. Tölgyesi, I. Gresits, T. Past, L. Szabó, G. Volent, and T. Pintér
An analytical procedure was developed for the alpha-spectrometric determination of uranium (238U, 235U, 234U) and transuranium isotopes (239+240Pu, 244Cm) in liquid radioactive wastes (sludges, evaporation residues) of low and medium activity from the VVER-440 reactor of the nuclear power plant in Paks, and in waste waters to be released into the environment. Radioactive sludge samples were separated to a liquid phase and a wet suspension (solid) phase by centrifuging, and the two phases were treated and analyzed separately, in different ways. A sample preparation procedure based on chemical separation was worked out for the isolation of the alpha-emitting components of radioactive evaporation residues which were saturated with boric acid. To the separate determination of the low activity alpha-emitting isotopes appearing in waste waters to be released into the environment, a microvawe concentration technique was applied. The accuracy of the results obtained using the above chemical separation and alpha-spectrometry was tested in the framework of the international U. S. DOE EML Quality Assessment Program.
Authors:N. Vajda, J. Larosa, T. Pintér, G. Keömley, D. Bódizs, and Zs. Molnár
A simple and effective method has been developed for the determination of the transuranium isotopes in primary coolant samples of WWER-440 type reactors. Membrane filters containing undissolved particles from about one liter coolant were destroyed by mineral acids, then americium-curium isotopes and neptunium-plutonium isotopes were coprecipitated sequentially with NdF3 based on their different redox behavior. Alpha-spectra were determined by silicon semiconductor detectors connected to multichannel analyzers. Activity ratios measured in a reactor unit with a failed fuel element were compared with calculated values of the same type of reactor under similar conditions. Parameters of the defective fuel (burnup, original enrichment) could be estimated.
Authors:G. Bognár, Cs. Pintér, B. Horváth, T. Sydo, E. Ligeti, J. Pulai, and D. Lőrinczy
Osteonecrosis (ON) of the femoral frequently occurs after steroid medication. One of the final pathways leading to steroid
induced ON is thought to be pathologic fat metabolism. The pathobiological mechanism underlying the induction of fat metabolism
outslides by steroids leading to ON has not been fully elucidated. The purpose of this study was to examine the intraoperative
obtained gluteal fat tissue from ON patients with histology, gas chromatography (GC) and differential scanning calorimetry
(DSC) and to compare them with otherwise healthy patient’s samples. The histological sections showed no significant differences
compared with the control group. GC revealed that fraction of saturated fatty acids decreased in ON samples from mean values
of controls of 24% to 21, the polyunsaturated fraction from 20 to 14%. The monounsaturated acids showed an increase from mean
rate of 52% of the controls to 65% of steroid treated samples. DSC curves correlate with chromatographic analysis of the tissue
fatty acids (Steroid treated, heating between 0–100°C: Tm=5.7°C, ΔH= −15.8J/g−1; heating between −20–100°C: Tm= −9.96 and 5.85°C, ΔH= −59.17 and −16.2 J g−1. Non-necrotic, heating between 0–100°C: two separable transition with Tm=5.7 and 9.9°C, total ΔH= −20.8 J g−1; heating between −20–100°C: Tm= −10.9 and 4.95°C, total ΔH= −75.8 J g−1.)
Our preliminary findings are rather tendentious. Further investigations are needed with higher sample rate and under other
anamnestic circumstances too.
Authors:H. K. Pintér, K. K. Boros, E. Pálfi, and G. Veres
In this study we assessed nutritional intake, body composition, and their relationship in patients with paediatric inflammatory bowel disease (IBD).
We conducted a longitudinal, prospective study of 38 patients' nutritional intake using 3-day food records (FR) and bioimpedance analysis of body composition. FR were evaluated by Nutricomp DietCAD software. Results were analysed with Microsoft Excel 2013 and IBM SPSS Statistics 22 software.
Patients treated with biological and conventional therapy (CT) had a higher intake of vegetable protein and carbohydrate from starch than those treated earlier with exclusive enteral nutrition (EEN) in the remission phase (F = 5.926, F = 5.130, P < 0.05). The former EEN group had a higher intake of iron compared to the other two groups (F = 3.967, P = 0.036). Protein intake and fat-free mass (FFM) had a significant positive correlation, while added sugar correlated with body fat mass (BFM) in the same way (R2 = 0.122, R2 = 0.169, P < 0.05). Body-fat mass in patients of the biological therapy (BT) group overstepped the healthy median, and the FFM in the EEN group stayed under it.
Our results confirm that it is essential to monitor body composition and not only measure body weight. Patients should be advised based on their body composition, therapy, and phase of the disease.
Authors:K.N. Hussein, L. Friedrich, R. Pinter, Cs. Németh, G. Kiskó, and I. Dalmadi
This study was conducted to evaluate the effect of bioactive compounds (BACs): linalool (LIN) and piperine (PIP) on chicken meat characteristics. The meat was treated with 500, 1000 ppm of BACs, vacuum packaged and stored at 4 °C for 8 days. Physicochemical characteristics, lipid oxidation (thiobarbituric acid reactive substances, TBARS), microbiological status, and sensorial (electronic-nose based) properties were investigated. Both BACs significantly increased the redness (a*) and chroma (C*) values in meat compared to increased lightness (L*) and higher TBARS in control. Although both BACs showed overlapping aroma profile, the E-nose was able to distinguish between the different meat groups. LIN with various dilution ratios, particularly 1:10 (v:v), showed in vitro growth inhibition against Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium, and Bacillus cereus, concomitantly Listeria monocytogenes required 1:80 (v:v) to be inhibited, and no inhibition was detected for Pseudomonas lundensis. In contrast, PIP at different dilutions did not exhibit inhibitory activity. Regarding aerobic mesophilic counts (AMC), less than 7 log CFU g−1 were recorded except for control showing higher log. Both BACs have potential to improve quality characteristics and increase the shelf life of meat and meat products.
Authors:Cs. Pintér, G. Bognár, B. Horváth, T. Sydo, E. Ligeti, J. Pulai, and D. Lőrinczy
Avascular necrosis of human femoral head (ANFH) causes incongruity in the joint that leads to disability in patients requires
total hip arthroplasty (THA). Several etiological factors of ANFH have been proposed in the literature but there are cases
of idiopathic origin. We observed macroscopic variation in quality of the subcutaneous fat tissue in patients with ANFH compared
to patients with osteoarthritis or hip fracture during THA procedures.
The samples were analysed by histology, gas chromatography (GC) and differential scanning calorimetry (DSC).
Conclusion: the alteration in the fatty acid profile did not cause histological changes, however we could detect biochemical
changes using DSC and GC.