Authors:M. Cantone, G. Gambarini, N. Molho, and L. Pirola
A method for Cr determination in biological samples based on proton nuclear activation is presented. The activation was induced by a 13.8 MeV proton beam of the AVF Cyclotron of Milan University via a (p, n) reaction on the nuclei of the target. For the quantitative determination Cd has been chosen as reference element. The method has been applied to Cr determination in human serum samples. The experimental procedure is described and results are presented and discussed.
Authors:R. Bassini, M. Cantone, D. De Bartolo, G. Gambarini, A. Giussani, and M. Malatesta
An apparatus for cyclic charged particle activation was designed, built and connected to the 7 MV Van de Graaff CN accelerator of the Laboratori Nazionali dell'Istituto Nazionale di Fisica Nucleare di Legnaro (Italy). It mainly consists of a two-sample transverse moving system controlled by a Cycle Control Module (CCM). This module is based on a Programmable Logic Device (PLD) for the definition of the requested flow-maps and functions. The CCM operates the cc motor for sample movements by means of a power width modulation technique. It also controls the beam switch and the setting mode of the acquisition system which is connected to two HPGe detectors. As a feasibility test, the best experimental conditions for aluminium determination in complex. Al-doped biological samples were found to be an irradiation time of 3.5 minutes and a measuring time of 6 minutes. The signal to background ratio is reported as a function of the number of cycles.
Authors:M. Cantone, D. De Bartolo, G. Gambarini, A. Giussani, N. Molho, L. Pirola, Ch. Hansen, E. Werner, and P. Roth
An investigation on tellurium metabolism by administration of stable tellurium isotopes124Te and126Te has been performed. Fractional intestinal absorption was determined in rabbits by the double tracer technique. The investigated subjects were given an enriched solution of one tellurium isotope orally and a few minutes later an enriched solution of the other isotope intravenously. The124Te and126Te contents in plasma samples were determined by proton nuclear activation. The methodology described offers a means to study tellurium metabolism in humans without radiation risk.
Authors:M. Cantone, D. De Bartolo, A. Giussani, N. Molho, L. Pirola, G. Gambarini, Ch. Hansen, P. Roth, and E. Werner
The feasibility of ruthenium metabolism studies by stable tracer administration, with a methodology based on proton nuclear activation, is presented. In order to test that the amount of stable tracer administered does not perturb significantly the mechanism investigated, a series of comparative experiments with administration of both radioactive and stable tracers has been performed on animals. As the most critical pathway seems to be the intravenous injection, four male rabbits were given an intravenous injection of radioactive106Ru. Successively, the rabbits were given either a further injection of radioactive106Ru or injection of different quantities of natural Ru. The activity of106Ru and the concentration of natural Ru were measured in plasma samples withdrawn at different time intervals from the injections and the results were compared. Some biokinetic parameters and tissue distribution of Ru in rabbits were determined.